Portable sequencing as a teaching tool in conservation and biodiversity research

PLoS Biol. 2020 Apr 16;18(4):e3000667. doi: 10.1371/journal.pbio.3000667. eCollection 2020 Apr.

Abstract

As biodiversity loss continues to accelerate, there is a critical need for education and biomonitoring across the globe. Portable technologies allow for in situ molecular biodiversity monitoring that has been historically out of reach for many researchers in habitat nations. In the realm of education, portable tools such as DNA sequencers facilitate in situ hands-on training in real-time sequencing and interpretation techniques. Here, we provide step-by-step protocols as a blueprint for a terrestrial conservation genetics field training program that uses low-cost, portable devices to conduct genomics-based training directly in biodiverse habitat countries.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biodiversity
  • Conservation of Natural Resources / methods*
  • DNA Barcoding, Taxonomic / instrumentation
  • DNA Barcoding, Taxonomic / methods
  • Ecosystem
  • Female
  • Genetics / education*
  • Genetics / instrumentation*
  • Genetics / organization & administration
  • Humans
  • Male
  • Peru
  • Polymerase Chain Reaction / instrumentation
  • Polymerase Chain Reaction / methods

Grants and funding

Field Projects International (https://fieldprojects.org) is a 501c3 accredited nonprofit organization that organized the two field programs that this publication references. Proceeds from the program fees travel, room and board at field stations for all authors (MW, GE, AP, and SP) during the programs, and compensated MW, GE, and AP for their time instructing on the first program. MW and GE serve on the board of directors of this nonprofit organization, but the financial arm of the organization that funded the program had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.