Conversion of liquid hot water, acid and alkali pretreated industrial hemp biomasses to bioethanol

In this work, four varieties of hemp biomasses (Helena, SS Beta, Tygra, and Elleta Campana) pretreated with liquid hot water (LHW), H₂SO₄, and NaOH were investigated for ethanol production. Physicochemical and morphological properties of the pretreated hemp biomass were characterized. LHW achieved high glucan (85-98%) and xylan (67-71%) recoveries. H₂SO₄ induced significant glucan decomposition (5.9-10.6 g/L) and inhibitor formation (4.5-7.4 g/L of HMF and 2.8-4.5 g/L of furfural) in resulting slurries. Both LHW and H₂SO₄ pretreatments resulted in low glucose and ethanol yields due to recondensed lignin units. NaOH pretreatment achieved high glucose and ethanol yields due to efficient lignin removal (58.6-75.3%). There was no significant variation in ethanol yield among the four hemp varieties pretreated by NaOH. H₂SO₄ and NaOH pretreated biomasses showed apparent terraced-field structures and microporous protuberances. Changes in crystallinity indexes and intensities of FTIR peaks were consistent with enhanced cellulose and decreased amorphous hemicellulose.