Abstract
Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in ∼30 minutes.
Publication types
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
Animals
-
Betacoronavirus / isolation & purification
-
COVID-19 Testing
-
Clinical Laboratory Techniques / methods
-
Coronavirus Infections / diagnosis
-
Herpesvirus 1, Equid / isolation & purification
-
Herpesvirus 4, Equid / isolation & purification
-
Horse Diseases / diagnosis*
-
Horse Diseases / microbiology
-
Horse Diseases / virology
-
Horses
-
Influenza A Virus, H3N8 Subtype / isolation & purification
-
Lab-On-A-Chip Devices*
-
Mobile Applications
-
Molecular Diagnostic Techniques / methods*
-
Nose / microbiology
-
Nose / virology
-
Nucleic Acid Amplification Techniques / methods*
-
Point-of-Care Systems
-
Respiration Disorders / diagnosis
-
Respiration Disorders / microbiology
-
Respiration Disorders / veterinary*
-
Respiration Disorders / virology
-
SARS-CoV-2
-
Smartphone*
-
Streptococcus equi / isolation & purification