Electrochemical Detection of Genomic DNA Utilizing Recombinase Polymerase Amplification and Stem-Loop Probe

Shirin Khaliliazar; Liangqi Ouyang; Andrew Piper; Georgios Chondrogiannis; Martin Hanze; Anna Herland; Mahiar Max Hamedi

doi:10.1021/acsomega.0c00341

Electrochemical Detection of Genomic DNA Utilizing Recombinase Polymerase Amplification and Stem-Loop Probe

ACS Omega. 2020 May 20;5(21):12103-12109. doi: 10.1021/acsomega.0c00341. eCollection 2020 Jun 2.

Authors

Shirin Khaliliazar¹, Liangqi Ouyang¹, Andrew Piper¹, Georgios Chondrogiannis¹, Martin Hanze¹, Anna Herland^{2

3}, Mahiar Max Hamedi¹

Affiliations

¹ Department of Fibre and Polymer Technology; School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Tekninkringen 56-58, SE-100 44 Stockholm, Sweden.
² Department of Micro and Nanosystem; School of Electrical Engineering and Computer Science, KTH Royal Institute of Technology, Malvinas Väg 10, SE 114 28, Stockholm Sweden.
³ AIMES, Advancement for Integrated Medical and Engineering Sciences, Department of Neuroscience, Karolinska Institute, SE 171 77, Stockholm Sweden.

Abstract

Nucleic acid tests integrated into digital point-of-care (POC) diagnostic systems have great potential for the future of health care. However, current methods of DNA amplification and detection require bulky and expensive equipment, many steps, and long process times, which complicate their integration into POC devices. We have combined an isothermal DNA amplification method, recombinase polymerase amplification, with an electrochemical stem-loop (S-L) probe DNA detection technique. By combining these methods, we have created a system that is able to specifically amplify and detect as few as 10 copies/μL Staphylococcus epidermidis DNA with a total time to result of 70-75 min.