The present work describes a dual-readout assay for the determination of an antipsychotic drug olanzapine using Rhodamine B modified silver nanoparticles (AgNPs). AgNPs, when mixed with Rhodamine B, quenched its fluorescence emission with high quenching efficiency as evident from the Stern Volmer plot. Transmission electron microscopy image and Dynamic Light Scattering histogram of Rhodamine B bound AgNPs showed a stable monodispersed nanosuspension. Addition of olanzapine to Rhodamine B-bound AgNPs resulted in reappearance of fluorescence, which was dependent on the amount of olanzapine added to the system. Besides displacing the surface bound Rhodamine B molecules, it caused aggregation of AgNPs which formed the basis of dual-readout sensor. Several parameters such as pH, reaction time and order of addition of the three components which may influence the analytical signal were studied and optimized. The method was validated for linearity, sensitivity, selectivity, accuracy, precision and recovery. Based on this dual-readout system, linear concentration range was established from 0.05 to 10 µM (fluorescence measurement) and 5.0 to 50 µM (colorimetric response) for olanzapine. The limit of detection (LOD) using fluorescence and colorimetric approach was 0.013 µM and 1.25 µM, respectively. The proposed method showed excellent selectivity for olanzapine in presence of several antipsychotic drugs, cations, sugars and amino acids. Finally, the method was successfully applied to a pharmacokinetic study of olanzapine in rats and also for analyzing pharmaceutical formulations.
Keywords: Fluorescence; Olanzapine; Pharmaceuticals; Pharmacokinetics; Rhodamine; Silver nanoparticles.