This study reports a microfluidic cell deformation-based method to deliver the Cas9 ribonucleoprotein (RNP) complexes to different cell types for efficient genome editing, including hard-to-transfect human primary CD4+ T cells. The RNP based CRISPR-Cas9 system has great advantage in shortening reaction time and reducing off-target problems, which holds great potential in future gene therapy applications.
Keywords: Cas9 ribonucleoprotein; cell deformation; delivery; gene therapy; microfluidics.
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.