Bovine Serum Albumin (BSA)/polyacrylonitrile (PAN) biohybrid nanofibers coated with a biomineralized calcium deficient hydroxyapatite (HA) shell for wound dressing

Shahin Homaeigohar; Ting-Yu Tsai; Eman S Zarie; Mady Elbahri; Tai-Horng Young; Aldo R Boccaccini

doi:10.1016/j.msec.2020.111248

Bovine Serum Albumin (BSA)/polyacrylonitrile (PAN) biohybrid nanofibers coated with a biomineralized calcium deficient hydroxyapatite (HA) shell for wound dressing

Mater Sci Eng C Mater Biol Appl. 2020 Nov:116:111248. doi: 10.1016/j.msec.2020.111248. Epub 2020 Jul 2.

Authors

Shahin Homaeigohar¹, Ting-Yu Tsai², Eman S Zarie³, Mady Elbahri⁴, Tai-Horng Young⁴, Aldo R Boccaccini⁵

Affiliations

¹ Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058, Erlangen, Germany; Nanochemistry and Nanoengineering, Department of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, Kemistintie 1, 00076 Aalto, Finland. Electronic address: shahin.homaeigohar@fau.de.
² Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan.
³ Nanochemistry and Nanoengineering, Department of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, Kemistintie 1, 00076 Aalto, Finland; Department of Therapeutical Chemistry, Pharmaceutical and Drug Industries Research Division National Research Centre, Dokki 12311, Giza, Egypt.
⁴ Nanochemistry and Nanoengineering, Department of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, Kemistintie 1, 00076 Aalto, Finland.
⁵ Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058, Erlangen, Germany.

PMID: 32806254
DOI: 10.1016/j.msec.2020.111248

Abstract

Here, for the first time, a nanofibrous (NF) wound dressing comprising biomineralized polyacrylonitrile (PAN) nanofibers is developed. In contrast to the majority of the currently available nanofibrous wound dressings that are based on natural polymers, PAN is a synthetic, industrial polymer, which has been rarely considered for this purpose. PAN NFs are first hydrolyzed to allow for tethering of biofunctional agents (here Bovine Serum Albumin (BSA)). Later, the biofunctionlized PAN NFs are biomineralized by immersion in simulated body fluid (SBF). As a result, core-shell, calcium deficient hydroxyapatite (HA)/BSA/PAN nanofibers form, that are mechanically stronger (elastic modulus; 8.5 vs. 6 MPa) compared to the untreated PAN NFs. The biomineralized PAN NFs showed promising bioactivity as reflected in the cell biology tests with fibroblast and keratinocyte cells. Hs68 fibroblasts and HaCat keratinocytes were found to be more viable in the presence of the biomineralized NFs than when they were co-cultured with the neat PAN NFs. Such mechanical and biological characteristics of the biomineralized PAN NFs are favorable for wound dressing applications.

Keywords: Biofunctionalization; Biomineralization; Nanofiber; Polyacrylonitrile (PAN); Wound dressing.

MeSH terms

Acrylic Resins
Bandages
Calcium
Durapatite
Nanofibers*
Serum Albumin, Bovine

Substances

Acrylic Resins
polyacrylonitrile
Serum Albumin, Bovine
Durapatite
Calcium