Abstract
This study examined the antibacterial effect of protoporphyrin IX-ethylenediamine derivative (PPIX-ED)-mediated photodynamic antimicrobial chemotherapy (PPIX-ED-PACT) against Pseudomonas aeruginosa in vitro and in vivo. PPIX-ED potently inhibited the growth of Pseudomonas aeruginosa by inducing reactive oxygen species production via photoactivation. Atomic force microscopy revealed that PPIX-ED-PACT induced the leakage of bacterial content by degrading the bacterial membrane and wall. As revealed using acridine orange/ethidium bromide staining, PPIX-ED-PACT altered the permeability of the bacterial membrane. In addition, the antibacterial effect of PPIX-ED-PACT was demonstrated in an in vivo model of P. aeruginosa-infected wounds. PPIX-ED (100 μM) decreased the number of P. aeruginosa colony-forming units by 4.2 log10. Moreover, histological analysis illustrated that the wound healing rate was 98% on day 14 after treatment, which was 10% higher than that in the control group. According to the present findings, PPIX-ED-PACT can effectively inhibit the growth of P. aeruginosa in vitro and in vivo.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Anti-Bacterial Agents / pharmacology
-
Anti-Bacterial Agents / therapeutic use*
-
Cell Membrane / drug effects
-
Cell Membrane / radiation effects
-
Ethylenediamines / chemistry
-
Ethylenediamines / pharmacology
-
Ethylenediamines / therapeutic use
-
Female
-
Light
-
Mice
-
Mice, Inbred BALB C
-
Microbial Viability / drug effects
-
Models, Biological
-
NIH 3T3 Cells
-
Photobleaching
-
Photochemotherapy*
-
Photosensitizing Agents / pharmacology
-
Photosensitizing Agents / therapeutic use
-
Protoporphyrins / chemistry
-
Protoporphyrins / pharmacology
-
Protoporphyrins / therapeutic use
-
Pseudomonas Infections / drug therapy*
-
Pseudomonas Infections / microbiology*
-
Pseudomonas aeruginosa / physiology*
-
Pseudomonas aeruginosa / radiation effects
-
Wound Healing / drug effects
-
Wound Infection / drug therapy*
-
Wound Infection / microbiology*
Substances
-
Anti-Bacterial Agents
-
Ethylenediamines
-
Photosensitizing Agents
-
Protoporphyrins
-
ethylenediamine
-
protoporphyrin IX
Grants and funding
This study was supported by the national Mega-project for Innovative Drugs (2019ZX09721001-006-001 to Ge Hong) and the medical and health science and technology innovation project of Chinese Academy of Medical Science (2019-I2M-1-005 to Ge Hong). The funder provided support in study design.