CRISPR Typing of Salmonella Isolates

Nikki Shariat; Edward Dudley

doi:10.1007/978-1-0716-0791-6_5

CRISPR Typing of Salmonella Isolates

Methods Mol Biol. 2021:2182:39-44. doi: 10.1007/978-1-0716-0791-6_5.

Authors

Nikki Shariat¹, Edward Dudley²

Affiliations

¹ Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, USA. nikki.shariat@uga.edu.
² Department of Food Science, College of Agricultural Sciences, The Pennsylvania State University, University Park, PA, USA.

PMID: 32894485
DOI: 10.1007/978-1-0716-0791-6_5

Abstract

Polymerase chain reaction (PCR) and sequencing-based subtyping tools are useful for rapid analyses of Salmonella isolates. Here we describe the process of clustered regularly interspaced short palindromic repeat-multiple virulence locus sequence typing (CRISPR-MVLST) for Salmonella subtyping.

Keywords: CRISPR-MVLST; PCR; Salmonella; Sequencing; Subtyping.

MeSH terms

Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
DNA, Bacterial / genetics
Multilocus Sequence Typing / methods*
Polymerase Chain Reaction / methods
Salmonella / genetics*
Salmonella / isolation & purification*
Salmonella Infections / microbiology*
Virulence / genetics

Substances

DNA, Bacterial