SWATH-MS-Based Proteomics: Strategies and Applications in Plants

Mo-Xian Chen; Youjun Zhang; Alisdair R Fernie; Ying-Gao Liu; Fu-Yuan Zhu

doi:10.1016/j.tibtech.2020.09.002

SWATH-MS-Based Proteomics: Strategies and Applications in Plants

Trends Biotechnol. 2021 May;39(5):433-437. doi: 10.1016/j.tibtech.2020.09.002. Epub 2020 Oct 6.

Authors

Mo-Xian Chen¹, Youjun Zhang², Alisdair R Fernie², Ying-Gao Liu³, Fu-Yuan Zhu⁴

Affiliations

¹ Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, China; International Cultivar Registration for Osmanthus, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, China; CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
² Center for Plant Systems Biology and Biotechnology, 4000 Plovdiv, Bulgaria; Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany.
³ State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Taian, Shandong, China. Electronic address: liuyg@sdau.edu.cn.
⁴ Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, China; International Cultivar Registration for Osmanthus, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, China. Electronic address: fyzhu@njfu.edu.cn.

PMID: 33036785
DOI: 10.1016/j.tibtech.2020.09.002

Abstract

Most applied proteomic approaches require labeling steps. Recent technological advances provide an alternative label-free proteomics approach: SWATH-MS. This powerful tool is now widely used in animal studies but has drawn far less attention in plants. Here we summarize how this promising technology can be applied to facilitate functional analysis in plant research.

Keywords: SWATH mass spectrometry; label-free proteomics; plants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Botany* / methods
Botany* / trends
Mass Spectrometry
Plants* / genetics
Proteome
Proteomics* / methods
Proteomics* / trends

Substances

Proteome