IRT1 and ZIP2 were involved in exogenous hydrogen-rich water-reduced cadmium accumulation in Brassica chinensis and Arabidopsis thaliana

Xue Wu; Nana Su; Xiaomeng Yue; Bo Fang; Jianwen Zou; Yahua Chen; Zhenguo Shen; Jin Cui

doi:10.1016/j.jhazmat.2020.124599

IRT1 and ZIP2 were involved in exogenous hydrogen-rich water-reduced cadmium accumulation in Brassica chinensis and Arabidopsis thaliana

J Hazard Mater. 2021 Apr 5:407:124599. doi: 10.1016/j.jhazmat.2020.124599. Epub 2020 Dec 13.

Authors

Xue Wu¹, Nana Su², Xiaomeng Yue³, Bo Fang⁴, Jianwen Zou⁵, Yahua Chen⁶, Zhenguo Shen⁷, Jin Cui⁸

Affiliations

¹ College of Life Sciences, Nanjing Agricultural University, Nanjing, China; The Agriculture Ministry Key Laboratory of Agricultural Engineering in the Middle and Lower Reaches of Yangtze River, Institute of Agricultural Facilities and Equipment, Jiangsu Academy of Agricultural Sciences, Nanjing, China. Electronic address: 2016216001@njau.edu.cn.
² College of Life Sciences, Nanjing Agricultural University, Nanjing, China. Electronic address: sunana@njau.edu.cn.
³ College of Life Sciences, Nanjing Agricultural University, Nanjing, China. Electronic address: 2018116010@njau.edu.cn.
⁴ College of Life Sciences, Nanjing Agricultural University, Nanjing, China. Electronic address: 2019816115@njau.edu.cn.
⁵ College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China. Electronic address: jwzou21@njau.edu.cn.
⁶ College of Life Sciences, Nanjing Agricultural University, Nanjing, China. Electronic address: yahuachen@njau.edu.cn.
⁷ College of Life Sciences, Nanjing Agricultural University, Nanjing, China. Electronic address: zgshen@njau.edu.cn.
⁸ College of Life Sciences, Nanjing Agricultural University, Nanjing, China. Electronic address: cuijin@njau.edu.cn.

PMID: 33360184
DOI: 10.1016/j.jhazmat.2020.124599

Abstract

The results of Cd (cadmium) concentration, Cd²⁺ fluorescent staining, NMT (non-invasive micro-test technology) analysis of Cd absorption revealed the remarkably positive role of HRW in reducing Cd uptake by root of pak choi seedlings. BcIRT1 (iron-regulated transporter 1) and BcZIP2 (zinc-regulated transporter protein 2) are the main Cd transporters in pak choi, but their roles in the process of HRW-reduced Cd uptake is still far from being answered. In this study, we specifically verified the function of IRT1 and ZIP2 in HRW-reduced Cd absorption in pak choi and Arabidopsis thaliana. Heterologous and homologous expression in Arabidopsis thaliana displayed that Cd concentrations in wild-type (Col-0) and transgenic A. thaliana of IRT1 and ZIP2 were significantly reduced by HRW, except for irt1- and zip2-mutant. NMT detection showed that HRW not only decreased Cd²⁺ influx in root of WT and transgenic lines, but also enhanced the competition between Zn and Cd. Taken together, the HRW-induced reduction of Cd accumulation in plants may be result from depressing the expression of BcIRT1 and BcZIP2 and affecting the preference of BcIRT1 and BcZIP2 in ion uptake.

Keywords: BcIRT1; BcZIP2; Cd; HRW; Ion absorption.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis Proteins* / genetics
Arabidopsis* / genetics
Arabidopsis* / metabolism
Brassica* / metabolism
Cadmium* / metabolism
Cation Transport Proteins* / genetics
Hydrogen / metabolism
Plant Roots / genetics
Plant Roots / metabolism
Plants, Genetically Modified
Water

Substances

Arabidopsis Proteins
Cation Transport Proteins
IRT1 protein, Arabidopsis
ZIP2 protein, Arabidopsis
Cadmium
Water
Hydrogen