Immunocytochemical Detection of ALK and ROS1 Rearrangements in Lung Cancer Cytological Samples

Diane Frankel; Elise Kaspi; Patrice Roll

doi:10.1007/978-1-0716-1278-1_12

Immunocytochemical Detection of ALK and ROS1 Rearrangements in Lung Cancer Cytological Samples

Methods Mol Biol. 2021:2279:157-164. doi: 10.1007/978-1-0716-1278-1_12.

Authors

Diane Frankel¹, Elise Kaspi², Patrice Roll²

Affiliations

¹ Aix Marseille Univ, APHM, INSERM, MMG, Hôpital la Timone, Service de Biologie Cellulaire, Marseille, France. diane.frankel@univ-amu.fr.
² Aix Marseille Univ, APHM, INSERM, MMG, Hôpital la Timone, Service de Biologie Cellulaire, Marseille, France.

PMID: 33683692
DOI: 10.1007/978-1-0716-1278-1_12

Abstract

The detection of molecular alterations such as ROS1 and ALK rearrangements is performed as part of the diagnosis of advanced-stage lung adenocarcinoma. These alterations allow the treatments with tyrosine kinase inhibitors. Cytological samples are very useful as up to 40% patients are diagnosed with this type of sample. Here we describe the immunocytochemistry technique usable to reveal the overexpression of ALK or ROS1 tyrosine kinase receptors secondary to ALK and ROS1 rearrangements, respectively.

Keywords: ALK; Adenocarcinoma; Cytology; Immunocytochemistry; Lung cancer; ROS1.

MeSH terms

Adenocarcinoma of Lung* / diagnosis
Adenocarcinoma of Lung* / enzymology
Adenocarcinoma of Lung* / genetics
Adenocarcinoma of Lung* / pathology
Anaplastic Lymphoma Kinase* / genetics
Anaplastic Lymphoma Kinase* / metabolism
Female
Gene Rearrangement*
Humans
Immunohistochemistry*
Lung Neoplasms* / diagnosis
Lung Neoplasms* / enzymology
Lung Neoplasms* / genetics
Lung Neoplasms* / pathology
Male
Protein-Tyrosine Kinases* / genetics
Protein-Tyrosine Kinases* / metabolism
Proto-Oncogene Proteins* / genetics
Proto-Oncogene Proteins* / metabolism

Substances

Proto-Oncogene Proteins
ALK protein, human
Anaplastic Lymphoma Kinase
Protein-Tyrosine Kinases
ROS1 protein, human