MiR-144/451a cluster synergistically modulates growth and metastasis of Oral Carcinoma

Objectives: MicroRNA (miRNA) clusters co-transcribe and function in a coordinated fashion mediating synergistic or antagonistic regulatory effects. MiR-144 and miR-451a are deregulated in various cancers but the combined regulatory role of miR-144/451a cluster in oral squamous cell carcinoma (OSCC) remains unexplored. In the present study, we studied the synergistic effect of miR-144/451a cluster on oral cancer progression.

Materials and methods: miR-144 and miR-451a expression was explored in OSCC cell lines by quantitative real-time PCR (qRT-PCR). Proliferation, wound healing, migration and invasion, spheroid formation, and colony formation assays were performed after transfection with miR-144-3p, miR-451a, miR-144-5p, and co-expressed miR-144/451a. Expression of putative target genes was analyzed using qRT-PCR and Western blotting.

Results: miR-144 and miR-451a were downregulated in all cell lines. The cell viability and stemness of cancer cell lines were unaltered when treated with miRNA mimics. However, co-expressed miR-144/451a significantly reduced the migratory, invasive, and clonogenic potential of cells than individual miRNAs.

Conclusion: miR-144/451a cluster functions as a tumor suppressor in OSCC by inhibiting cancer cell invasion, migration, and clonogenic potential.