Asthma as chronic airway disease has high prevalence in children and imbalance of Th1/Th2 is a critical mechanism in pathogenesis of the asthma. Baicalein as a cell protective and anti-inflammatory flavonoid may have anti-asthma effect. Therefore, for better using lung, baicalein was used in chitosan-nanoparticle as anti-asthma treatment. Baicalein was loaded and encapsulated in chitosan nanoparticle. The morphology, physical characters (particle size, zeta potential and FT-IR) were analyzed. Drug encapsulation and loading capacity, accumulative release-time were studied. After asthma model producing, the mice were treated with L-B-NP and E-B-NP. At least, MCh challenge test, Cytokines measurement and Lung Histopathology were done. Nanoparticles had average size 285 ± 25 nm with negative charge -2.5 mV. The L-B-NP decreased penh value and E-B-NP decreased inflammation. Both nanoparticles increased IL-12 and decreased IL-5. Also, L-B-NP decreased mucus secretion in bronchi. L-B-NP and E-B-NP control immune-allergo-inflammatory response of asthma. L-B-NP controlled AHR and E-B-NP controlled inflammation that can be used as controlling anti-asthma drug.
Keywords: AB, alcian blue; AHR, airway hyperresponsiveness; AP-1, activator protein 1; Airway; BALf, bronchoalveolar lavage fluid; BBB, blood–brain barrier; COX, cyclooxygenase; E-B-NP, encapsulated-Baicalein-nanoparticles; ELISA, the enzyme-linked immunosorbent assay; FT-IR, fourier-transform infrared spectroscopy; Flavonoid; H&E, hematoxylin and eosin; IL, interleukin; IP, intraperitoneal; IT, intratracheal; Inflammation; L-B-NP, loaded-Baicalein-nanoparticles; MAP, mitogen-activated protein; MCh, methacholine; MTT, The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide; NF-κB, the nuclear factor-κB; Nano; OVA, ovalbumin; PAS, periodic acid–schiff; PG, prostaglandin; TNF, tumor necrosis factor; Th, T lymphocyte helper; iNOS, inducible nitric oxide synthase; mV, millivolt; nm, nanometer.
© 2021 The Author(s).