A nanocellulose-based film with excellent antimicrobial and antioxidant activity was developed by adding Pickering emulsion which was stabilized by functionalized particles. First, TOCNC-g-PEI nanoparticles were prepared by grafting polyethyleneimine (PEI) onto TEMPO-oxidized cellulose nanocrystals (TOCNCs) and used to stabilize oregano essential oil (OEO) Pickering emulsions. The contact angle of TOCNC-g-PEI nanoparticles was 79.7°, and the minimum inhibitory concentration against L.monocytogenes and E.coli was 0.50 mg/mL. Second, the emulsion droplets were stably dispersed in cellulose nanofibril (CNF) suspensions owing to the depletion stability of CNFs. Finally, the films were dried and emulsion droplets formed oil core/CNF shell microcapsules in active CNF films and completely encapsulated OEO in active CNF films. The inhibition rates of the film against L.monocytogenes and E.coli were 97.28% and 97.23%, respectively. The influence of Pickering emulsion on the active CNF films was discussed. The developed active CNF films have promising application in food preservation and active packaging.
Keywords: Active packaging; Antimicrobial nanoparticles; Cellulose nanofibril; Pickering emulsion; Polyethyleneimine (PEI).
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