Effect of Pretreatment on Detection of 37 Pesticide Residues in Chrysanthemum indicum

Xiao-Ying Lu; Yan-Qin Ouyang; Wei-Ya Zeng; Cui-Qing Lin; Lu-Hua Xiao; Gui-Hua Luo; Ruo-Ting Zhan; Ping Yan

doi:10.1155/2021/8854025

Effect of Pretreatment on Detection of 37 Pesticide Residues in Chrysanthemum indicum

J Anal Methods Chem. 2021 Dec 9:2021:8854025. doi: 10.1155/2021/8854025. eCollection 2021.

Authors

Xiao-Ying Lu^{1

2

3}, Yan-Qin Ouyang⁴, Wei-Ya Zeng¹, Cui-Qing Lin^{1

2

3}, Lu-Hua Xiao^{1

2

3}, Gui-Hua Luo^{1

2

3}, Ruo-Ting Zhan^{1

2

3}, Ping Yan^{1

2

3}

Affiliations

¹ School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
² Key Laboratory of Chinese Medicinal Resources from Lingnan (Guangzhou University of Chinese Medicine), Ministry of Education, Guangzhou 510006, China.
³ Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines, Guangzhou 510006, China.
⁴ China Resources Sanjiu Medical & Pharmaceutical Co.,Ltd., Shenzhen 518110, China.

Abstract

This study aimed to develop a method, followed by gas chromatography-mass spectrometry, for detecting 37 pesticides in Chrysanthemum indicum (C. indicum) and investigating the decrease in the matrix-induced enhancement effect. The influence of QuEChERS extraction and matrix solid-phase dispersion (MSPD) on the recovery and matrix effect (ME) was compared. extraction and matrix solid-phase dispersion (MSPD) on the recovery and matrix effect (ME) was compared to decrease the ME. The cleanup sorbents, volume and type of solvent, and treatment time were optimized. The accuracy (as recovery), precision (as relative standard deviation, RSD), linearity, limit of quantitation, and limit of detection were determined. The recoveries at the three levels using mixed standard solution ranged between 76% and 120% with RSD ≤15%, and 76% and 120% with RSD ≤11% for MSPD and QuEChERS extraction, respectively. The results suggested that the ME for 21 pesticides was in the range of 80%-120% after MSPD and 15% after QuEChERS extraction. QuEChERS extraction was simpler and faster than MSPD. This methodology was applied in the analysis of 27 C. indicum samples; phorate was most frequently detected (63.0% of the sample).