Objective: To investigate the expression of Talin1 in the fallopian tube and chorionic villi in patients with tubal pregnancy and its role in regulating invasion and migration of trophoblasts.
Methods: Immunohistochemistry and Western blotting were used to detect the localization and expression level of Talin1 in the fallopian tube and chorionic villi in patients with tubal pregnancy and in women with normal pregnancy. In the cell experiment, HTR-8/SVneo cells was transfected with Talin1 siRNA and the changes in cell invasion and migration were assessed using scratch assay and Transwell assay. The expressions of MMP-2, MMP-9, N-cadherin and Snail in the transfected cells were detected by qRT-PCR and Western blotting.
Results: Positive expression of Talin1 was detected in both normal fallopian tube tissues and tissues from women tubal pregnancy, and its expression was localized mainly in the cytoplasm of cilia cells. The expression level of Talin1 was significantly higher in both the fallopian tube and chorionic villi in women with tubal pregnancy than in normal fallopian tube and chorionic villi samples (P < 0.01). In HTR-8/SVneo cells, transfection with Talin1 siRNA significantly inhibited cell invasion (P < 0.01) and migration (P < 0.05), down-regulated the expression of N-cadherin, MMP-2 and Snail (P < 0.05), and up-regulated the expression of MMP-9 in the cells (P < 0.05).
Conclusion: The expression of Talin1 in the fallopian tube and chorionic villi is significantly increased in women with tubal pregnancy, suggesting the association of Talin1-regulated trophoblast cell invasion with the occurrence of tubal pregnancy.
目的: 验证Talin1在妊娠输卵管组织及输卵管妊娠绒毛中的表达差异及对HTR-8/SVneo细胞侵袭、迁移的影响，探讨其作用机制。
方法: 采用免疫组化及Western blot检测Talin1在妊娠输卵管组织及输卵管妊娠绒毛中的表达定位及蛋白表达差异；HTR-8/SVneo细胞内转染Talin1 siRNA，划痕实验及Transwell实验检测Talin1对HTR-8/SVneo细胞侵袭、迁移的影响；qRTPCR及Western blot检测HTR-8/SVneo细胞中MMP-2、MMP-9、N-cadherin、Snail的表达。
结果: 正常输卵管组织及妊娠输卵管组织中均可检测到Talin1的阳性表达，主要表达于纤毛细胞的细胞质中；妊娠输卵管组织中Talin1的蛋白表达高于正常输卵管组织（P < 0.01）；输卵管妊娠绒毛中Talin1表达高于宫内妊娠绒毛（P < 0.01）；沉默Talin1抑制HTR-8/SVneo细胞的侵袭（P < 0.01）和迁移（P < 0.05）；Talin1下调HTR-8/SVneo细胞中N-cadherin、MMP-2、Snail的表达（P < 0.05），上调MMP-9的表达（P < 0.05）。
结论: 输卵管妊娠患者的输卵管组织及绒毛中Talin1的表达显著升高，输卵管妊娠的发生可能和Talin1调控妊娠滋养细胞的侵袭相关。
Keywords: Talin1; invasion; tubal pregnancy.