Abstract
The simultaneous assay of the reverse transcriptase and 60S to 70S RNA of oncogenic RNA viruses is described. The virus can be detected at low concentrations in biological fluids containing enzymatically active cell fragments or other contaminants. The fact that two features diagnostic of the oncornaviruses are used in the tests increases the certainty with which a positive outcome can be interpreted.
MeSH terms
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Alpharetrovirus / analysis
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Animals
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Autoradiography
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Centrifugation, Density Gradient
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DNA, Viral / biosynthesis
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Edetic Acid
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Female
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Humans
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Mammary Tumor Virus, Mouse / analysis
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Mammary Tumor Virus, Mouse / enzymology
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Mice
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Milk / analysis
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Molecular Weight
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Mycoplasma / analysis
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Oncogenic Viruses / analysis*
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Oncogenic Viruses / enzymology
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RNA Viruses / analysis*
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RNA Viruses / enzymology
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RNA, Viral / analysis*
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Rauscher Virus / analysis
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Retroviridae / analysis
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Ribonucleases / pharmacology
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Tritium
Substances
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DNA, Viral
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RNA, Viral
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Tritium
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Edetic Acid
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Ribonucleases