Abstract
The pep4-3 mutation results in a 90-95% reduction in the levels of five vacuolar hydrolases in yeast, including proteinases A and B, carboxypeptidase Y, RNase(s) and the repressible alkaline phosphatase. The mutation is without effect on two secreted glycoproteins, on an enzyme of the vacuolar membrane, and on a proteinase located outside of the vacuole. Mutations at the PEP4 locus exhibit a dosage effect on the levels of some, but not all, of the enzymes whose expression requires the function of the gene.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alkaline Phosphatase / genetics
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Carboxypeptidases / genetics
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Cytoplasmic Granules / enzymology
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Genes
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Genes, Fungal*
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Genes, Regulator
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Glycoside Hydrolases / genetics
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Peptide Hydrolases / genetics*
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Protein Processing, Post-Translational
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Ribonucleases / genetics
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Saccharomyces cerevisiae / genetics*
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beta-Fructofuranosidase
Substances
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Ribonucleases
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Alkaline Phosphatase
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Glycoside Hydrolases
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beta-Fructofuranosidase
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Carboxypeptidases
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Peptide Hydrolases