Abstract
A simple microassay for the determination of UDP-glucuronic acid was developed on the basis of the formation of benzo[a]pyrene 3-glucuronide catalysed by UDP-glucuronyltransferase of guinea-pig liver. As little as 1-5 pmol of UDP-glucuronic acid was detectable in extracts of heat-denatured probes of liver or cultured cells equivalent to 10-50 micrograms of cellular protein.
MeSH terms
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Animals
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Benzopyrenes
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Cells, Cultured
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Glucuronosyltransferase
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Guinea Pigs
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Male
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Mice
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Microchemistry / methods
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Microsomes, Liver / analysis
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Rats
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Spectrometry, Fluorescence
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Uridine Diphosphate Glucuronic Acid / analysis*
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Uridine Diphosphate Sugars / analysis*
Substances
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Benzopyrenes
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Uridine Diphosphate Sugars
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Uridine Diphosphate Glucuronic Acid
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3-hydroxybenzo(a)pyrene
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Glucuronosyltransferase