Abstract
NF-kappa B is a transcription factor composed of the p50 and p65 subunits. Recent works identified another human gene which encodes a molecule related to the p50 subunit, termed p50B, p49 or lyt-10. Here, we isolated the cDNA clones encoding chicken p50B/p97 (Lyt-10). The deduced amino acid sequence of the precursor protein, p97, shows conservation of the overall structure, and 86% identity in the Rel homology domain (RHD) and 56% identity in the ankyrin repeat domain (ARD) to human p50B/p97. Expression of this gene is highest in the chicken spleen.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Ankyrins / genetics
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Base Sequence
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Blotting, Northern
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Chickens / genetics*
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Conserved Sequence
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DNA Primers
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DNA, Complementary / isolation & purification
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Gene Expression
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Humans
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Molecular Sequence Data
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NF-kappa B / biosynthesis
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NF-kappa B / genetics*
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NF-kappa B p52 Subunit
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Poly A / analysis
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Poly A / metabolism
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Polymerase Chain Reaction
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Protein Precursors / genetics
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins c-rel
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RNA / analysis
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RNA / metabolism
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RNA, Messenger
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Repetitive Sequences, Nucleic Acid
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Sequence Homology, Amino Acid
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Spleen / metabolism
Substances
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Ankyrins
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DNA Primers
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DNA, Complementary
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NF-kappa B
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NF-kappa B p52 Subunit
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Protein Precursors
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-rel
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RNA, Messenger
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Poly A
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RNA