A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome

R R Samaha; R Green; H F Noller

doi:10.1038/377309a0

A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome

Nature. 1995 Sep 28;377(6547):309-14. doi: 10.1038/377309a0.

Authors

R R Samaha¹, R Green, H F Noller

Affiliation

¹ Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA.

PMID: 7566085
DOI: 10.1038/377309a0

Abstract

Interaction of the conserved CCA terminus of tRNA with rRNA in the peptidyl transferase P site has been studied by in vitro genetics. A watson-Crick G-C pair between G2252 in a conserved hairpin loop of 23S rRNA and C74 at the acceptor end of tRNA is required for proper functional interaction of the CCA end of tRNA with the ribosomal P site. These findings establish a direct role for 23S rRNA in protein synthesis.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Base Composition
Base Sequence
Conserved Sequence
Escherichia coli
Molecular Sequence Data
Mutagenesis
Nucleic Acid Conformation
Peptidyl Transferases / metabolism*
RNA, Bacterial / metabolism
RNA, Ribosomal, 23S / genetics
RNA, Ribosomal, 23S / metabolism*
RNA, Transfer / metabolism*
Ribosomes / metabolism*

Substances

RNA, Bacterial
RNA, Ribosomal, 23S
RNA, Transfer
Peptidyl Transferases