Abstract
The CorA Mg2+ transport system is the dominant constitutive uptake mechanism in Salmonella typhimurium and Escherichia coli. Southern blot hybridization and PCR techniques were used to screen a panel of 18 additional gram-negative bacterial species for corA homologs. Virtually all strains tested positive for the presence of corA. Thus, corA appears to be ubiquitous within gram-negative bacteria and is likely their major Mg2+ influx system.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Proteins / genetics*
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Biological Transport / genetics
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Blotting, Southern
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Carrier Proteins / genetics*
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Cation Transport Proteins*
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DNA Primers
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Gram-Negative Bacteria / classification
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Gram-Negative Bacteria / genetics*
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Magnesium / metabolism*
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Polymerase Chain Reaction
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Species Specificity
Substances
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Bacterial Proteins
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Carrier Proteins
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Cation Transport Proteins
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CorA protein, Salmonella
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DNA Primers
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Magnesium