Abstract
The inhibitory effect of milk in the PCR detection of Listeria monocytogenes could be overcome by washing the contaminated milk sample with phosphate-buffered saline and concentrating the bacteria to 1/10 of the original volume. In order to avoid a possible failure in the detection of virulent L. monocytogenes, a one-step procedure which enabled demonstration of three virulence-associated genes, prfA, hlyA, and plcB, simultaneously in a single PCR mixture was developed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Proteins / genetics
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Base Sequence
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DNA, Bacterial / analysis
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Escherichia coli Proteins*
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Food Microbiology*
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Genes, Bacterial*
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Hemolysin Proteins / genetics
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Listeria monocytogenes / genetics
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Listeria monocytogenes / isolation & purification
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Listeria monocytogenes / pathogenicity*
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Milk / microbiology*
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Molecular Sequence Data
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Peptide Termination Factors
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Polymerase Chain Reaction / methods*
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Trans-Activators
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Type C Phospholipases / genetics
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Virulence
Substances
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Bacterial Proteins
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DNA, Bacterial
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Escherichia coli Proteins
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Hemolysin Proteins
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Hlya protein, E coli
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Peptide Termination Factors
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Trans-Activators
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Type C Phospholipases
Associated data
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GENBANK/X12157
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GENBANK/X59723