Trichorzins HA and MA, antibiotic peptides from Trichoderma harzianum. II. Sequence determination

S Hlimi; S Rebuffat; C Goulard; S Duchamp; B Bodo

doi:10.7164/antibiotics.48.1254

Trichorzins HA and MA, antibiotic peptides from Trichoderma harzianum. II. Sequence determination

J Antibiot (Tokyo). 1995 Nov;48(11):1254-61. doi: 10.7164/antibiotics.48.1254.

Authors

S Hlimi¹, S Rebuffat, C Goulard, S Duchamp, B Bodo

Affiliation

¹ Laboratoire de Chimie des Substances Naturelles, URA 401 CNRS, Muséum National d'Histoire Naturelle, Paris, France.

PMID: 8557565
DOI: 10.7164/antibiotics.48.1254

Abstract

A series of 18-residue antibiotic-antifungal peptides, trichorzins HA and MA, were isolated from Trichoderma harzianum strains exhibiting antagonistic properties against phytopathogenic fungi. The sequences of the nine major pure peptides isolated by HPLC were determined by positive ion FAB-MS data and two-dimensional NMR measurements, including COSY, HOHAHA, ROESY and 1H-13C LRCOSY experiments.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Anti-Bacterial Agents / chemistry*
Chromatography, High Pressure Liquid
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Peptides / chemistry*
Sequence Analysis*
Sequence Homology
Spectrometry, Mass, Fast Atom Bombardment
Trichoderma / chemistry*

Substances

Anti-Bacterial Agents
Peptides