Abstract
A novel, probably chromosomally encoded, aminoglycoside phosphotransferase gene was cloned on a 2,996-bp PstI fragment from Pseudomonas aeruginosa and designated aph (3')-IIb. It coded for a protein of 268 amino acids that showed 51.7% amino acid identity with APH (3')-II [APH(3') is aminoglycoside-3' phosphotransferase] from Tn5. Two other open reading frames on the cloned fragment showed homology to a signal-transducing system in P. aeruginosa.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aminoglycosides
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Anti-Bacterial Agents / pharmacology
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Cloning, Molecular
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DNA, Bacterial / analysis
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DNA, Bacterial / isolation & purification
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Drug Resistance, Microbial / genetics
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Escherichia coli / genetics
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Genes, Bacterial / genetics*
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Kanamycin Kinase
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Molecular Sequence Data
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Open Reading Frames
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Phosphotransferases (Alcohol Group Acceptor) / genetics*
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Plasmids / analysis
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Pseudomonas aeruginosa / enzymology
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Pseudomonas aeruginosa / genetics*
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Sequence Homology, Nucleic Acid
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Signal Transduction
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Transduction, Genetic
Substances
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Aminoglycosides
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Anti-Bacterial Agents
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DNA, Bacterial
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Phosphotransferases (Alcohol Group Acceptor)
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Kanamycin Kinase