Abstract
Ethanol treatment of etiolated maize seedlings led to a marked enhancement in metabolism of the herbicides metolachlor and prosulfuron. cDNA clones representing eight genes that encode putative cytochrome P450 enzymes were isolated from maize. They fall into three families and are designated CYP71C5, CYP73A6, A7, and A8, and CYP81A1, A2, A3, and A4. Ethanol treatment induced the CYP81A subfamily at the mRNA level in both roots and shoots of etiolated seedlings.
MeSH terms
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Acetamides / metabolism
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Base Sequence
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Captan / pharmacology
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Cytochrome P-450 Enzyme System / biosynthesis*
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Cytochrome P-450 Enzyme System / genetics*
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DNA, Plant / biosynthesis
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Enzyme Induction / drug effects
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Ethanol / pharmacology*
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Fungicides, Industrial / pharmacology
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Gene Expression Regulation, Enzymologic / drug effects*
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Gene Expression Regulation, Plant / drug effects*
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Herbicides / metabolism
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Microsomes / metabolism
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Molecular Sequence Data
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Polymerase Chain Reaction
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RNA, Messenger / biosynthesis
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RNA, Plant / biosynthesis
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Zea mays / enzymology*
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Zea mays / genetics*
Substances
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Acetamides
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DNA, Plant
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Fungicides, Industrial
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Herbicides
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RNA, Messenger
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RNA, Plant
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Ethanol
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Cytochrome P-450 Enzyme System
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Captan
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metolachlor