Abstract
Although cytokinin plays a central role in plant development, little is known about cytokinin signal transduction. Five Arabidopsis thaliana mutants that exhibit typical cytokinin responses, including rapid cell division and shoot formation in tissue culture in the absence of exogenous cytokinin, were isolated by activation transferred DNA tagging. A gene, CKI1, which was tagged in four of the five mutants and induced typical cytokinin responses after introduction and overexpression in plants, was cloned. CKI1 encodes a protein similar to the two-component regulators. These results suggest that CKI1 is involved in cytokinin signal transduction, possibly as a cytokinin receptor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Arabidopsis / genetics
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Arabidopsis / physiology*
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Arabidopsis Proteins*
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Cloning, Molecular
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Cytokinins / pharmacology
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Cytokinins / physiology*
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DNA, Bacterial / genetics
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DNA, Complementary / genetics
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DNA, Plant / genetics
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Ethylenes / metabolism
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Genes, Plant
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Histidine Kinase
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Molecular Sequence Data
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Mutation
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Open Reading Frames
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Plant Proteins / chemistry
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Plant Proteins / metabolism
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Polymorphism, Restriction Fragment Length
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Protein Kinases / chemistry
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Protein Kinases / genetics
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Protein Kinases / physiology*
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Receptors, Cell Surface*
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Sequence Homology, Amino Acid
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Signal Transduction*
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Transformation, Genetic
Substances
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Arabidopsis Proteins
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Cytokinins
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DNA, Bacterial
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DNA, Complementary
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DNA, Plant
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Ethylenes
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Plant Proteins
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Receptors, Cell Surface
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T-DNA
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ethylene receptors, plant
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ethylene
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Protein Kinases
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Histidine Kinase
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CKI1 protein, Arabidopsis