Abstract
GlcNAc beta 1-2Man and GlcNAc beta 1-6Man were synthesized using the reverse hydrolysis activity of beta-N-acetylglucosaminidase from both jack beans and Bacillus circulans. In turn, Gal beta 1-4GlcNAc beta 1-2Man and Gal beta 1-4GlcNAc beta 1-6Man were synthesized regioselectively using the transglycosylation activity of beta-galactosidase from Diplococcus pneumoniae and B. circulans, respectively. These di- and trisaccharides are important components of complex type sugar chains and will be used as intermediates in our synthetic studies.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylglucosaminidase / metabolism
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Bacillus / enzymology
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Carbohydrate Sequence
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Chromatography, High Pressure Liquid
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Disaccharides / chemical synthesis*
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Disaccharides / metabolism*
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Glycosylation
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Hydrolysis
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Magnetic Resonance Spectroscopy
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Molecular Sequence Data
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Streptococcus pneumoniae / enzymology
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Trisaccharides / chemical synthesis*
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Trisaccharides / metabolism*
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beta-Galactosidase / metabolism
Substances
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Disaccharides
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Trisaccharides
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N-acetylglucosaminyl-1-2-mannopyranose
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galactopyranosyl-1-4-N--acetylglucosaminyl-1-2-mannopyranose
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beta-Galactosidase
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Acetylglucosaminidase