Abstract
Neural activity and neurotrophins induce synaptic remodeling in part by altering gene expression. A cDNA encoding a glycosylphoshatidylinositol-anchored protein was identified by screening for hippocampal genes that are induced by neural activity. This molecule, named neuritin, is expressed in postmitotic-differentiating neurons of the developing nervous system and neuronal structures associated with plasticity in the adult. Neuritin message is induced by neuronal activity and by the activity-regulated neurotrophins BDNF and NT-3. Purified recombinant neuritin promotes neurite outgrowth and arborization in primary embryonic hippocampal and cortical cultures. These data implicate neuritin as a downstream effector of activity-induced neurite outgrowth.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies
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Base Sequence
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Brain / metabolism*
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CHO Cells
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Cerebral Cortex / metabolism
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Cricetinae
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DNA, Complementary
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Embryo, Mammalian
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GPI-Linked Proteins
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Glycosylphosphatidylinositols / metabolism
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Hippocampus / drug effects
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Hippocampus / metabolism
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Humans
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In Situ Hybridization
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Molecular Sequence Data
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Nerve Growth Factors / pharmacology*
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Neurites / drug effects
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Neurites / physiology*
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Neurons / drug effects
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Neurons / metabolism*
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Neuropeptides / biosynthesis*
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Neuropeptides / isolation & purification
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Neuropeptides / pharmacology*
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Peptide Fragments / chemistry
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Peptide Fragments / immunology
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RNA, Messenger / biosynthesis
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Rats
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / pharmacology
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Transcription, Genetic*
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Transfection
Substances
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Antibodies
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DNA, Complementary
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GPI-Linked Proteins
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Glycosylphosphatidylinositols
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NRN1 protein, human
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Nerve Growth Factors
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Neuropeptides
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Nrn1 protein, rat
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Peptide Fragments
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RNA, Messenger
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Recombinant Proteins