Glycerophosphorylcholine (GPC) was incorporated as the chain extender in a series of poly(tetramethylene oxide)-based polyurethane block copolymers. In order to determine the feasibility of use of these polyurethanes in biomedical devices, the effects of GPC incorporation on physical properties were studied. The effect of soft-segment molecular weight was also investigated. Biocompatibility of these materials was studied with regard to bacterial adhesion and protein deposition. Tensile testing showed that as GPC content increased, elongation at break decreased, while Young's modulus increased. Differential scanning calorimetry (DSC) results showed slightly decreased glass transition temperatures (Tgs) with increasing GPC content, indicating increased phase separation. Dynamic mechanical analysis (DMA) confirmed the decrease in Tg and the increase in rubbery plateau modulus with increasing GPC content. Water absorption was also increased with GPC content. Decreased bacterial adhesion was found on the GPC-containing materials compared to other functionalized polyurethanes. These experiments were carried out in a radial flow chamber utilizing automated video microscopy. Bacterial attachment was found to be lower on the GPC-containing polyurethanes both in the absence of and after pre-adsorption with plasma proteins.