We evaluated the use of dual-energy x-ray absorptiometry (DXA) for measuring the gross body composition of small subjects in 97 pigs that ranged from 5 to 27 kg live body weight. Scans were performed using a Lunar DPXL densitometer in the pediatric mode (Version 3.8e). The DXA scans of the live pigs provided measurements of total fat, lean, and bone mineral content. After scanning, the pigs were killed, the entire body was ground, and samples were analyzed chemically (CHEM) for fat, protein, ash, and water content. We found that DXA significantly underestimated the percentage of fat in the body (DXA, 6.9+/-.33% vs CHEM, 10.9+/-.31%, P < .001). The correlation (r) between DXA and chemical measures of percentage fat was .86 and for grams of fat it was .96. Lean tissue mass measured by DXA was highly correlated with CHEM measurements of total grams of body water (r = .99), total grams of body protein (r = .94), and lean body mass (r = .99). The average DXA bone mineral content was within 2% of the amount estimated from total body ash and the correlation between the two values was .94. The relationships between DXA and CHEM measurements for percentages of body composition of pigs that weighed between 5 and 27 kg are described by the following regression equations: %fatCHEM = 5.22 + [.817.fatDXA], (r = .86, standard error of the estimate, SEE = 1.56); %proteinCHEM = -7.8 + [.256%leanDXA], (r = .35, SEE = 2.3); %waterCHEM = -5.2 + [.808-%leanDXA], (r = .59, SEE = 3.67). These results are consistent with previously reported results and suggest that even though direct use of DXA readings may not be sufficiently accurate, the high degree of correlation indicates that with proper calibration the DXA values can be used to predict body composition.