Identification of an efflux pump gene, pmrA, associated with fluoroquinolone resistance in Streptococcus pneumoniae

M J Gill; N P Brenwald; R Wise

doi:10.1128/AAC.43.1.187

Identification of an efflux pump gene, pmrA, associated with fluoroquinolone resistance in Streptococcus pneumoniae

Antimicrob Agents Chemother. 1999 Jan;43(1):187-9. doi: 10.1128/AAC.43.1.187.

Authors

M J Gill¹, N P Brenwald, R Wise

Affiliation

¹ Division of Immunity and Infection, University of Birmingham Medical School, Birmingham B15 2TT, United Kingdom. m.j.gill@bham.ac.uk

Abstract

An open reading frame (ORF) homologous to norA was insertionally inactivated with cat in a fluoroquinolone-resistant pneumococcus with an efflux phenotype; this inactivation caused reversion to drug sensitivity. The ORF product has 24% amino acid sequence identity each to NorA and Bmr, which suggests that it is a major facilitator system pump of the 12-transmembrane-segment class.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Anti-Infective Agents / pharmacology*
Bacterial Proteins / genetics*
Drug Resistance, Microbial
Fluoroquinolones
Genes, Bacterial*
Molecular Sequence Data
Open Reading Frames
Polymerase Chain Reaction
Streptococcus pneumoniae / drug effects*
Streptococcus pneumoniae / genetics*

Substances

Anti-Infective Agents
Bacterial Proteins
Fluoroquinolones
pmrA protein, Bacteria

Associated data

GENBANK/AJ007367