Microfluidic system is widely employed in the detection of environmental contaminants and biological specimens. One of the critical issues which limits the applications of microfluidic chips is the limit of detection of trace specimens. Liquid⁻liquid extraction is of great importance in the preprocessing in microfluidic devices. In this paper, we developed a real-time fluorescence detection microfluidic chip combined with a microstructure-enhanced liquid⁻liquid laminar extraction technique, which concentrated the trace compound and realized real-time monitoring. Auxiliary microstructures integrated in the microfluidic chip were applied to increase the extraction efficiency, which was proved by the FEM (finite element method) simulation as well. A common fluorescence probe, Rhodamine 6G (Rh6g), was used in the experiment to demonstrate the performance of the microfluidic system. It revealed that the liquid⁻liquid laminar extraction combined with auxiliary microstructures of a cross shape was an effective method for enrichment. The efficiency of microstructure-enhanced liquid⁻liquid extraction was increased by 350% compared to the traditional laminar flow extraction.
Keywords: fluorescence detection; liquid–liquid extraction; lithography; microfluidic chip.