3N9Y,3MZS,3DAX,4LXJ,6CIR,3GPH


Conserved Protein Domain Family
p450

?
pfam00067: p450 
Click on image for an interactive view with Cn3D
Cytochrome P450
Cytochrome P450s are haem-thiolate proteins involved in the oxidative degradation of various compounds. They are particularly well known for their role in the degradation of environmental toxins and mutagens. They can be divided into 4 classes, according to the method by which electrons from NAD(P)H are delivered to the catalytic site. Sequence conservation is relatively low within the family - there are only 3 absolutely conserved residues - but their general topography and structural fold are highly conserved. The conserved core is composed of a coil termed the 'meander', a four-helix bundle, helices J and K, and two sets of beta-sheets. These constitute the haem-binding loop (with an absolutely conserved cysteine that serves as the 5th ligand for the haem iron), the proton-transfer groove and the absolutely conserved EXXR motif in helix K. While prokaryotic P450s are soluble proteins, most eukaryotic P450s are associated with microsomal membranes. their general enzymatic function is to catalyze regiospecific and stereospecific oxidation of non-activated hydrocarbons at physiological temperatures.
Statistics
?
PSSM-Id: 395020
View PSSM: pfam00067
Aligned: 50 rows
Threshold Bit Score: 248.348
Threshold Setting Gi: 117304
Created: 11-May-2020
Updated: 7-Aug-2020
Structure
?
Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
3N9Y_A    12 PSPGD-NGWLNLYHFWReTg---THKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGpnpERflIPPWV 87  human
Q02318    61 PRLGQ-LRFFFQLFVQG-Y----ALQLHQLQVLYKAKYGPMWMSYLGPQMHVNLASAPLLEQVMRQEGkypVRndMELWK 134 human
P15539    42 PQYSR-NKWLKMIQILReQg---QENLHLEMHQVFRELGPIFRHSVGKTQIVSVMLPEDAEKLHQVESmlpRRmhLEPWV 117 house mouse
P15150    42 PRCPG-NKWMRMLQIWKeQs---SENMHLDMHQTFQELGPIFRYDVGGRHMVFVMLPEDVERLQQADShhpQRmiLEPWL 117 cattle
Q07217    50 PGLWR-NGLANLYSFWKlDg---FRNIHRVMVHNFNTFGPIYREKIGYYDSVNIIKPEMPAILFKAEGhypKRltVEAWT 125 rainbow trout
3MZS_D    14 PSPGD-NGWLNLYHFWReKg---SQRIHFRHIENFQKYGPIYREKLGNLESVYIIHPEDVAHLFKFEGsypERydIPPWL 89  cattle
P28649    48 PGPG----YCLGIGPLIsHgrflWMGIGSACNYYNKMYGEFMRVWISGEETLIISKSSSMFHVMKHSHy-iSRf-GSKRG 121 house mouse
P19098    47 PGPG----YCMGIGPLIsHgrflWMGVGNACNYYNKTYGEFVRVWISGEETFIISKSSSVFHVMKHWNy-vSRf-GSKLG 120 chicken
P38364    51 PGPFLaRISRVWEIKKAaT-----GNIHEIVMDLHRCHGPIVRIGPNRYDFDTMEALKIIYRIGNALPk--ADyyIPFGL 123 [Nectria] haematoco...
EHA28363  36 PAPGLaAFTNFWLLLQTrR-----GHRFVVVDNAHKKYGKLVRIAPRHTSIADDG---AIQAVYGHGNgflKSdfYDAFV 107 Aspergillus niger A...
3N9Y_A    88 AYHQYYQRPIGVLLKKSAAWKKDRVALNQEVMAPe--ATknFLPLLDAVSRDFVSVLHRRIKKAGsgnySGDISDDLFRF 165 human
Q02318   135 EHRDQHDLTYGPFTTEGHHWYQLRQALNQRLLKPaeaAL--YTDAFNEVIDDFMTRLDQLRAESAsgnqVSDMAQLFYYF 212 human
P15539   118 AHRELRGLRRGVFLLNGPEWRLNRLRLNRNVLSPk--AVqkFVPMVDMVARDFLETLKEKVLQNArgslTMDVQQSLFNY 195 house mouse
P15150   118 AYRQARGHKCGVFLLNGPQWRLDRLRLNPDVLSLp--ALqkYTPLVDGVARDFSQTLKARVLQNArgslTLDIAPSVFRY 195 cattle
Q07217   126 SYRDYRNRKYGVLLKNGEDWRSNRVILNREVISPk--VLgnFVPLLDEVGQDFVARVHKKIERSGqdkwTTDLSQELFKY 203 rainbow trout
3MZS_D    90 AYHRYYQKPIGVLFKKSGTWKKDRVVLNTEVMAPe--AIknFIPLLNPVSQDFVSLLHKRIKQQGsgkfVGDIKEDLFHF 167 cattle
P28649   122 LQCIGMHENGIIFNNNPSLWRTIRPFFMKALTGP---GLvrMVEVCVESIKQHLDRLGEVTDTSG----YVDVLTLMRHI 194 house mouse
P19098   121 LQCIGMYENGIIFNNNPAHWKEIRPFFTKALSGP---GLvrMIAICVESTIVHLDKLEEVTTEVG----NVNVLNLMRRI 193 chicken
P38364   124 PSSPNLFDVQNPARHS-AMKKQVASLYTMTALLS-------YEAGVDGQTIILKEQLQRFCDQKQ----VIDLPQFLQYY 191 [Nectria] haematoco...
EHA28363 108 SIHRGLFNTRDRAEHTRKRKTVSHTFSMKSIGQFeqyIHgnIELFVKQWNRMADTQRNPKTGFAS-----LDALNWFNYL 182 Aspergillus niger A...
3N9Y_A   166 AFESITNVIFGERQGmLEEVV-----------NPEAQRFIDAIYQ--MFHTSVPMLNLPPDl--FRLFRTKTWKDHVAAW 230 human
Q02318   213 ALEAICYILFEKRIGcLQRSI-----------PEDTVTFVRSIGL--MFQNSLYATFLPKWtrpVLPFWKRYLDGWNAIF 279 human
P15539   196 TIEASNFALFGERLGlLGHDL-----------SPGSLKFIHALHS--MFKSTSQLLFLPKSl--TRWTSTRVWKEHFDAW 260 house mouse
P15150   196 TIEASTLVLYGERLGlLTQQP-----------NPDSLNFIHALEA--MLKSTVQLMFVPRRl--SRWMSTNMWREHFEAW 260 cattle
Q07217   204 ALESVGSVLYGERLGlMLDYI-----------NPEAQHFIDCISL--MFKTTSPMLYIPPAm--LRRVGAKIWRDHVEAW 268 rainbow trout
3MZS_D   168 AFESITNVMFGERLGmLEETV-----------NPEAQKFIDAVYK--MFHTSVPLLNVPPEl--YRLFRTKTWRDHVAAW 232 cattle
P28649   195 MLDTSNMLFLGIP---LDES------------A--IVKKIQGY-------FNAWQALLIKPn--IFFKISWLYRKYERSV 248 house mouse
P19098   194 MLDTSNKLFLGVP---LDES------------A--IVLKIQNY-------FDAWQALLLKPd--IFFKISWLCKKYEEAA 247 chicken
P38364   192 AFDVIGVITVGKSMGmMETNSdt-------ngACGALDAMWHYSSmmAFIPHMHAWWLRLSs--LLPIDVPIKGLTEYVE 262 [Nectria] haematoco...
EHA28363 183 AFDIIGDLAFGAPFGmLDKGKdfaemrktpdsPPSYVQAVEVLNRrgEVSATLGCYPALKPf--AKYLPDSFFRDGIQAV 260 Aspergillus niger A...
3N9Y_A   231 DVIFSKADIYTQNFYWELRQKgsVHHDYRGILYRLLGd----sKMSFEDIKANVTEMLAGGVDTTSMTLQWHLYEMARNL 306 human
Q02318   280 SFGKKLIDEKLEDMEAQLQAAgpDGIQVSGYLHFLLAs----gQLSPREAMGSLPELLMAGVDTTSNTLTWALYHLSKDP 355 human
P15539   261 DVISEYANRCIWKVHQELRLG--SSQTYSGIVAELISq----gSLPLDAIKANSMELTAGSVDTTAIPLVMTLFELARNP 334 house mouse
P15150   261 DYIFQYANRAIQRIYQELALG--HPWHYSGIVAELLMr----aDMTLDTIKANTIDLTAGSVDTTAFPLLMTLFELARNP 334 cattle
Q07217   269 DGIFNQADRCIQNIYRTMRQDtnTHGKYPGVLASLLMl----dKLSIEDIKASVTELMAGGVDTTSITLLWTLYELARHP 344 rainbow trout
3MZS_D   233 DTIFNKAEKYTEIFYQDLRRKt-EFRNYPGILYCLLKs----eKMLLEDVKANITEMLAGGVNTTSMTLQWHLYEMARSL 307 cattle
P28649   249 KDLKDEIAVLVEKKRHKVSTAekLEDCMDFATDLIFAerr--gdLTKENVNQCILEMLIAAPDTMSVTLYFMLLLVAEYP 326 house mouse
P19098   248 KDLKGAMEILIEQKRQKLSTVekLDEHMDFASQLIFAqnr--gdLTAENVNQCVLEMMIAAPDTLSVTLFIMLILIADDP 325 chicken
P38364   263 QRIIQYRLKAAEFGDDDALKG-----ENNFLAKLILMer--qgTVTSTETQQAVGLNIGAGSDTTANALSSILYFLYTNP 335 [Nectria] haematoco...
EHA28363 261 EDLAGIAVARVNERLRPEVMA--NNTRVDLLARLMEGkdsngeKLGRAELTAEALTQLIAGSDTTSNTSCAILYWCMRTP 338 Aspergillus niger A...
3N9Y_A   307 KVQDMLRAEVLAAR-HQaqGDMATMLQLVPLLKASIKETLRLHPISV-TLQRYLVN---DLVLRDYMIPAKTLVQVAIYA 381 human
Q02318   356 EIQEALHEEVVGVVpAGq-VPQHKDFAHMPLLKAVLKETLRLYPVVP-TNSRIIEK---EIEVDGFLFPKNTQFVFCHYV 430 human
P15539   335 DVQKALRQESLAAE-ASiaANPQKAMSDLPLLRAALKETLRLYPVGG-FLERILSS---DLVLQNYHVPAGTLVLLYLYS 409 house mouse
P15150   335 EVQQAVRQESLVAE-ARisENPQRAITELPLLRAALKETLRLYPVGItLE-REVSS---DLVLQNYHIPAGTLVKVLLYS 409 cattle
Q07217   345 DLQEELRAEVAVAR-QStqGDMLQMLKMIPLVKGALKETLRLHPVAVsLQ-RYITE---EIVIQNYHIPCGTLVQLGLYA 419 rainbow trout
3MZS_D   308 NVQEMLREEVLNAR-RQaeGDISKMLQMVPLLKASIKETLRLHPISVtLQ-RYPES---DLVLQDYLIPAKTLVQVAIYA 382 cattle
P28649   327 EVEAAILKEIHTVV-GDr-DIKIEDIQNLKVVENFINESMRYQPVVD-LVMRRALE---DDVIDGYPVKKGTNIILNIGR 400 house mouse
P19098   326 TVEEKMMREIETVM-GDr-EVQSDDMPNLKIVENFIYESMRYQPVVD-LIMRKALQ---DDVIDGYPVKKGTNIILNIGR 399 chicken
P38364   336 RTLRRLREELDTHVkED--PIRFQQSQSMPYLQAVIKEALRLHPGVGtQLTRVVPKg--GLVIEGQFFPEGAEVGVNGWA 411 [Nectria] haematoco...
EHA28363 339 GVIEKLHKALDEAIpQDvdVPTHAMVKDIPYLQWVIWETMRIHSTSAmGLPREIPAgnpPVTISGHTFYPGDVVSVPSYT 418 Aspergillus niger A...
3N9Y_A   382 LGREPTFF-FDPENFDPTRWLSKDK----N-ITYFRNLGFGWGVRQCLGRRIAELEMTIFLINMLENFRVEIQHLSDVGT 455 human
Q02318   431 VSRDPTAF-SEPESFQPHRWLRNSQpatpRiQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGELK 509 human
P15539   410 MGRNPAVF-PRPERYMPQRWLERKR--------SFQHLAFGFGVRQCLGRRLAEVEMMLLLHHILKTFQVETLRQEDVQM 480 house mouse
P15150   410 LGRNPAVF-ARPESYHPQRWLDRQG----S-GSRFPHLAFGFGVRQCLGRRVAEVEMLLLLHHVLKNFLVETLEQEDIKM 483 cattle
Q07217   420 MGRDPDVF-PRPEKYLPSRWLRTEN-------QYFRSLGFGFGPRQCLGRRIAETEMQLFLIHMLENFRVDKQRQVEVHS 491 rainbow trout
3MZS_D   383 MGRDPAFF-SSPDKFDPTRWLSKDK----D-LIHFRNLGFGWGVRQCVGRRIAELEMTLFLIHILENFKVEMQHIGDVDT 456 cattle
P28649   401 MHR-LEYF-PKPNEFTLENFEKN--------VPYRYFQPFGFGPRGCAGKYIAMVMMKVVLVTLLRRFQVKTLQKRCIEN 470 house mouse
P19098   400 MHK-LEFF-PKPNEFSLENFEKN--------VPSRYFQPFGFGPRGCVGKFIAMVMMKAILVTLLRRCRVQTMKGRGLNN 469 chicken
P38364   412 LYHNKAIFgNDASVFRPERWLETKGn-----LNIGGSFAFGAGSRSCIGKNISILEMSKAIPQIVRNFDIEINHGDMTWK 486 [Nectria] haematoco...
EHA28363 419 IHRSKEIWgPDAEQFVPERWDPARLt----pRQKAAFIPFSTGPRACVGRNVAEMELLVICGTVFRLFEFEMQQEGPMET 494 Aspergillus niger A...
3N9Y_A   456 TFNL--ILMPEKPISFTF 471 human
Q02318   510 SVARi-VLVPNKKVGLQF 526 human
P15539   481 AYRF--VLMPSSSPVLTF 496 house mouse
P15150   484 VYRF--ILMPSTLPLFTF 499 cattle
Q07217   492 TFEL--ILLPEKPILLTL 507 rainbow trout
3MZS_D   457 IFNL--ILTPDKPIFLVF 472 cattle
P28649   471 IPKKndLSLHPNEDRHLV 488 house mouse
P19098   470 IQKNndLSMHPIERQPLL 487 chicken
P38364   487 NECWw-FVKPEYKAMIKP 503 [Nectria] haematococca mpVI
EHA28363 495 REG---FLRKPLGLQVGM 509 Aspergillus niger ATCC 1015
| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap