Conserved Protein Domain Family
GlnD_UR_UTase

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pfam08335: GlnD_UR_UTase 
GlnD PII-uridylyltransferase
This is a family of bifunctional uridylyl-removing enzymes/uridylyltransferases (UR/UTases, GlnD) that are responsible for the modification (EC:2.7.7.59) of the regulatory protein P-II, or GlnB (pfam00543). In response to nitrogen limitation, these transferases catalyze the uridylylation of the PII protein, which in turn stimulates deadenylylation of glutamine synthetase (GlnA). Deadenylylated glutamine synthetase is the more active form of the enzyme. Moreover, uridylylated PII can act together with NtrB and NtrC to increase transcription of genes in the sigma54 regulon, which include glnA and other nitrogen-level controlled genes. It has also been suggested that the product of the glnD gene is involved in other physiological functions such as control of iron metabolism in certain species. The region described in this family is found in many of its members to be C-terminal to a nucleotidyltransferase domain (pfam01909), and N-terminal to an HD domain (pfam01966) and two ACT domains (pfam01842).
Statistics
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PSSM-Id: 311992
View PSSM: pfam08335
Aligned: 93 rows
Threshold Bit Score: 88.007
Threshold Setting Gi: 81556393
Created: 21-Jul-2016
Updated: 4-Aug-2016
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 57012807  287 REMKQKISREVLRRNLQD--------NIKLGAGGIREIEFIVQTFQMIRggrdkilqqrsLLQVLPHLVTLNLLTKQQAK 358
gi 91206758  187 EFFQAKLEEQEKRHKRYNdtlyqlepNIKESPGGLRDIQTILWVAKRHF-----------GASSLQELMQHNFISLQEYK 255
gi 91206746  190 QFFLAKQAEQITRHHKYHdtaynlepNLKEGPGGLRDIQMIQWVSKRYF-----------NTWSFDSLLQHDFLTVSERK 258
gi 123345598 181 AFFAAKWQEQTTRHKRFNdteynlepDLKNAPGGLRDVQMIAWVAKRHF-----------NTDSLEALVSNGFLTPEEYG 249
gi 81170610  174 TFFDAKMEEQAKRHAQHNntesnlepDIKNAPGGIRDMNQIGWIAKRHF-----------RVNRIYDLVHLGFITEYELK 242
gi 81170620  164 qyFFAKLQEQQNRYAKYGetaynlepNIKNGPGGLRDLQILLSISKRHF-----------KIKKLAEGIGYGFITDKEYE 232
gi 81170623  177 AFFEAKMAEQRIRYSKYHntaynlepNVKEGPGGLRDIQLIGWIIRRHS-----------DARGLQDLVAHGWLTDAEYR 245
gi 500138254 185 AFFEAKLAEQQARHAKFHdtaynlepNIKSSPGGLRDIQMVGWVAKRHF-----------GAEHLHERVAGGFLTEAEYQ 253
gi 640084227 160 SFLVEKQKEQHNRHQNLSntaynlepNIKQSPGGLRDIQTIVWVAKWYF-----------DVNTLFELVDKQYLTTTEYE 228
gi 500095253 177 RFFTVKKEEQIKRHKNCRgdgynlepDLKNGSGGLRDIQTIAWIAKRHF-----------GVNSLIELTGSNYITQGEYR 245
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 57012807  359 HLGDAYIFHRQLENVLQAIDDQQTQSLPADakNQARIMYACQsywqkdqqnqplmieyhW-------QSWQQ---FLSTL 428
gi 91206758  256 EIQAAYLYLNRIRFALHRLKKRHEDRLLFD--HQQQLAELLN-----------------HddrpehnDSIKAveaFMKPY 316
gi 91206746  259 ELLESQSFLWQLRYGLHTLTGRREDRLLFD--HQIALAKQLG-----------------Yhd----qGPHLAveqLMKDY 315
gi 123345598 250 VLRKCLDYLWEVRWQLHVQTERNENRLLFD--HQRQLADRLG-----------------Hen----sSANLAvehFMKDF 306
gi 81170610  243 VLEEAESFLWEIRHHLHLLSKRDENRLLFD--HQREIAAKFG-----------------Ytra-egqPVNFAveqFMKRY 302
gi 81170620  233 ELKYCQNFLWRVRFALHMLAGKPEERLSFD--YQVKLAQFFG-----------------Yqd----qSHILAieqFMKDY 289
gi 81170623  246 ELKEAQAFLWRIRFALHALTGRCEDRLLFD--YQRELAGLFG-----------------Yrg----eTSNEVvegFMQDY 302
gi 500138254 254 ALHAGQCFLWDVRFGLHLIAGRREDRLLFD--HQVGLAELFG-----------------Yvd----dDQTLAveqFMQRY 310
gi 640084227 229 LLKISQLFLFKVRFALHIIANRREDRLAFQ--YQKSVATMLG-----------------Yv-----dGDSLSveaFMKDY 284
gi 500095253 246 ELVDCQAMLWTIRFALHSVTNRPDNRLLFD--YQTEVARMLG-----------------Ys-----gSGNTAieqMMKAF 301
                        170
                 ....*....|....*
gi 57012807  429 AQHQQHVRVIFNQLI 443
gi 91206758  317 YQNAHIVARLNEILL 331
gi 91206746  316 YRTAENTGRLNEMLL 330
gi 123345598 307 YRVALALSVLNEMLL 321
gi 81170610  303 YRTAQQVSTLNEMLL 317
gi 81170620  290 FKVIKRNRELNEMLL 304
gi 81170623  303 FRTVTGVERLNELLL 317
gi 500138254 311 FRTAMDISRLNEMLL 325
gi 640084227 285 YQTVTRVSRLNDILL 299
gi 500095253 302 YQTIHRVKELNEMLL 316
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