Conserved Protein Domain Family
Gln_amidase

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pfam15644: Gln_amidase 
Papain fold toxin 1, glutamine deamidase
A papain fold toxin domain found in bacterial polymorphic toxin systems. In these systems they might function either as a releasing peptidase or toxin. In Shigella flexneri, UniProtKB:Q8VSD5, this protein is expressed from a plasmid, and delivered into the host via the type III secretion system where it deamidates the glutamine residue at position 100 in ubiquitin-activating enzyme E2, UBC13, to a glutamic acid residue. Invasion of host cells by pathogens normally invokes an acute inflammatory response through activating the TRAF6-mediated signalling pathway. UBC13 helps to activate TRAF6. Thus deamidation of UBC13 results in the dampening of the inflammatory response. The key glutaminase deamidase activity is mediated by a cys-his-glu triad, present in all members of the family.
Statistics
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PSSM-Id: 317950
View PSSM: pfam15644
Aligned: 31 rows
Threshold Bit Score: 47.4231
Threshold Setting Gi: 902769510
Created: 29-Jul-2016
Updated: 4-Aug-2016
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 522167637  1605 NCLDCSLAALSSF-YGDPQVAQPrW-PDIRV--------GGSFETELGEDSGLTRAEEWLGGIWAGGSAGLpaaptaraa 1674
gi 524758135   350 NCQAAVAVHEARL-RGLNVTALG----YDDR--------KGSVSYELGEH-FESIWQHPKTGKTPKPTVLR--------- 406
gi 524232357   316 NCATCAPTYTLRL-RGFDIKAKG----RIKG--------SGSINDYIAHQHSFEAWKNIDGTPAVPVLTQEwmvrk---g 379
gi 524283769   311 NCQSCVVVHEARL-RGLDITALG----YSNK--------KDSVQFQLGEH-FEDAWINPKTGKVPSPTIIK--------- 367
gi 332698831   396 NCQSCVVSYEARL-RGYDVMARP-Y-----G--------ADDIMDELATH-TNLAWIDPVTGKHPEYIYDDk-------- 451
gi 1028045330  899 NCTRTPGAYMDRL-NGIDSTADP-L-----------------LVHEMDSRGTLEHVEERFGGQFSDR------------- 946
gi 497630995   876 NCSRCVRAVDLGF-TGTPASALP-W-PEGPGwglssntfADYARSLGADPAAFRQV------------------------ 928
gi 573020040   235 NCLSCATAVADTLkEGQLHCADP-TlRGGSP--------SQFSTLSHAEGG----------------------------- 276
gi 523979926   325 NCQTCAPAYALRL-RGFDITAKGn------T--------PGSKLEYLSRGRAFEVWKNADGTPAQHTSINdwlrs---kn 386
gi 511271149  1842 NCTRTPGAYMDRL-NGIDSTADP------------------LLVHEMDSRGTLEHVEERFGGQFSDR------------- 1889
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 522167637  1675 avsD-QYARLYQRV--A--Q--SGPGSAALVVADWLGAddvtgelildeeggvtpdGAHAFILV-YPSGvdRPVWWDPQH 1746
gi 524758135   407 ---GkSFDDMFGKV--EttT--KATGRYHIGINMPGNK-------------------GHVITAErFSDG--RIMFYDAQN 458
gi 524232357   380 ykqM-TPARYAEYF--NesC--REEGVYILTIGWKEGG-------------------GHATILQrFSDG--SLKYIEPQS 433
gi 524283769   368 ---G-G--DLIGKAekQ--M--KSVGRYHVGINYNAv-------------------EGHILTAEkTADE--MLYLYDAQS 416
gi 332698831   452 --iD-TAKKFLKFL--E--EnvEKDKRYTLQFSWKGKSr-----------------mGHIVSLDrDENN--LLRIYDPQC 505
gi 1028045330  947 ---A-DYDTVIREM--R--G--MPTDHHAVVAVKYLDEng--------------veRGHVAMVA-NTRD--GVAFIDPQS 999
gi 497630995   929 ---S-SYDDIIGDM--T--A--RGEGARGMVYIGRPGS-------------------AHVFNVV-HDQN--GVVFLDGQT 976
gi 573020040   277 -----MFDSLATMLdkA--N--AQPQCNAVLCVKRPRSvwrrl---------fsptDGHACNVV-KVDQ--TLFLVDAQK 335
gi 523979926   387 ylkM-TSKRYMEFF--NevC--KDEGVYELSIGWKGGG-------------------GHATILQrFKNG--ELRYIEPQS 440
gi 511271149  1890 ---A-DYDTVIREM--R--G--MPTDHHAVVAVKYLDEngv--------------erGHVAMVAnTRDG---VAFIDPQS 1942

                   .
gi 522167637  1747 G 1747
gi 524758135   459 G 459
gi 524232357   434 f 434
gi 524283769   417 G 417
gi 332698831   506 G 506
gi 1028045330 1000 G 1000
gi 497630995   977 G 977
gi 573020040   336 R 336
gi 523979926   441 D 441
gi 511271149  1943 G 1943
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