Conserved Protein Domain Family
selB

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TIGR00475: selB 
selenocysteine-specific elongation factor SelB
In prokaryotes, the incorporation of selenocysteine as the 21st amino acid, encoded by TGA, requires several elements: SelC is the tRNA itself, SelD acts as a donor of reduced selenium, SelA modifies a serine residue on SelC into selenocysteine, and SelB is a selenocysteine-specific translation elongation factor. 3-prime or 5-prime non-coding elements of mRNA have been found as probable structures for directing selenocysteine incorporation. This model describes the elongation factor SelB, a close homolog rf EF-Tu. It may function by replacing EF-Tu. A C-terminal domain not found in EF-Tu is in all SelB sequences in the seed alignment except that from Methanococcus jannaschii. This model does not find an equivalent protein for eukaryotes. [Protein synthesis, Translation factors]
Statistics
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PSSM-Id: 129567
View PSSM: TIGR00475
Aligned: 4 rows
Threshold Bit Score: 582.988
Threshold Setting Gi: 2833515
Created: 7-Oct-2014
Updated: 23-Jan-2015
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2822107     1 MIIATAGHVDHGKTTLLQAITGVN---ADRLPEEK-KRGMTIDLGYAYWPQPDg-RVPGFIDVPGHEKFLSNMLAGVGGI 75
gi 1173423     1 MIIVTSGHVDHGKTALLKALTGTS---TAHLPEEK-KRGMTIDLGYAYLPLEN--KVLGFIDVPGHEKFLSNMLAGLGGV 74
gi 2833515    10 VNVGLFGHIDHGKTQLAKQLTEIAstsALDKPKESqKRGITIDLGFSSFTLDR--YRITLVDAPGHSELIRTAIGAGNII 87
gi 499183102   4 ILFATAGHVDHGKTTLIKTLTGID---TDRLPEEK-KRGLSIDIGFAYIDFPDinTRLEIIDVPGHERFIKNAIAGICSA 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2822107    76 DHALLVVACDDGVMAQTREHLAILQLTGNPMLTVALTKADRVDEArvdEVERQVKEVLREYGFA-EAKLFITAATEGRGM 154
gi 1173423    75 HYAMLIVAADEGVAVQTKEHLAILRQLQFHEIIVVITKADRTNSA---QIESLIQTIKQDYSFLrNANYFVTSAETGQGI 151
gi 2833515    88 DAALLVVDAKEGPKTQTGEHLLVLDLLNIP-TIVVINKIDIANDEeikRTEMFMKQILNSTINLkNSKIIKISAKTGEGI 166
gi 499183102  80 SGLILVVDPNEGIMPQTIEHLRVAKSFGIKHGIAVLTKMDKVDEElahIAEEELIAFLEKEEMN--MEIVKVSAVTGQGI 157
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2822107   155 DALREHLLQLPEREHAs---QHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTgVNKPMRVRALHAQNQPTETA 231
gi 1173423   152 SELRHYLANLAELADT----QKPFRYAIDRVFSVKGAGTVVTGTAFSGTVKVNDEIYLS-TGQKIRIKAIHAQNTSSEQG 226
gi 2833515   167 GELKKELKNLLDSLDIkrdiNSYLKMPIDHAFKIKGVGTVVTGTIHKGKVEVGDNLRILpINHEVKVKSIQCFKQDVSIA 246
gi 499183102 158 EDLKNSIKKLLESINNln-kHKPLRIFVDSAFVVKGYGTVLRGSCFEGEVKEGDKVVVEpIGVISRVRKMQNHGVFVKKA 236
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2822107   232 NAGQRIALNIAgDAEKEQINRGdwLLADVPPEPFTRVIVELQTHTPLTQWQPLHIHHAASHVTGRVSLLEDN-------- 303
gi 1173423   227 IAGQRLALNLNaDLDRTPMKRGdwLLQNEPLPPTDRISVQILAEVPLNESQPVHIYHGASRTTGKLTLLQGKnaakndrt 306
gi 2833515   247 YAGDRVGMALM-GVEPESLFRG---CILTSEDTKLKVVDKFIAKVKILELFKYNLAPKMKVHIN---------------- 306
gi 499183102 237 VAGERIALNLP-EVDAKKVKRG--FLILKPESYEKSNVLIVKTEIDLKPGKIYQVFFGMRETVGKISVIDKG-------- 305
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2822107   304 LAELVFDTPLWLADNDRLVLRDiSARNTLAGARVVMLnpPRRGKRKpeylQWLASLARAQSDADALSVHLERGAVNLAdF 383
gi 1173423   307 LAEIILDSPLFLAFGDKLILRSgDTKTLIAGARVLEInsPKRHKRTevrlNFLANLALAENASQRIALTLQHNATTARqL 386
gi 2833515   307 IGLLTVPATIIPYKIEKINDKE-------------EP--------------IILEEIKGGDSCYCIFKLEERVVVDEG-- 357
gi 499183102 306 IYLVRLKENAIVRRGDKLVVLD-SSGNFLGGAEVLHP--KVRVTKK----AFIKKNIKDLLENFECYLLKERGPIGLK-L 377
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2822107   384 AWARQLNGEGMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATyhEQHrDEPGPGRERLRRMALPMedEALVLLLIE 463
gi 1173423   387 MWTEQLTSLQLDKALAERDAVRYQDWCFNPNYVQEKTQQILTALNIyhEQHnDQLGVSKARLYRMATLNqpENLIHHFID 466
gi 2833515   358 ------DKILIMRLDLPPTTLRICGFGEVIDFGEVEVKKIVVKEGK------VVKKKDKIYIEGLASSK-------TAGE 418
gi 499183102 378 EFFKRITGVSPKVANLKPESIEIRGVYYLKGFIENLKLKIKKFLDT--ELQ-NAFGVDKEKVKSMFSLN--EELLKYILD 452
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2822107   464 KMREsgdIHSHHGWLHLPDHKAGFSEEQQaIWQKAEPLFGDE---PWWVRDLAKETGTDEQAMRLTLRQAAQQGIITAIV 540
gi 1173423   467 EMLDdgrLQQTRGWIHLPEHKIQFNTEEKsRWTDVLNEFEKAngqAIWVRDMANALAIDESIMRNFMYKAGKLGYLTPIV 546
gi 2833515   419 KLIG-------G-KVYIPDKN---------IWGVIKGTFGTK---GALIAEFDEEVNGGEKVVLKRVRKWG--------- 469
gi 499183102 453 ELK----TYKIVNELIIDERKSDLEKNED--FQKLMSILKGG---IKEEREIILEGIPKEILTLSIKRKYAHRIGEYLII 523
                        570       580       590       600       610       620
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2822107   541 KDRYYRndrivefaNMIRDLDQECGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRD 607
gi 1173423   547 KDRFFLtetlyayaRLIKQIAEEKGKVSVNEVRDKLNFGRKLTVQLMEYFDRMGFLRRKGNDHILRD 613
gi 2833515       -------------------------------------------------------------------
gi 499183102 524 SDELLK--------KYINELKELGKTFNVQQAKNKLGLTRKYLIPLLEYLDYLGLTVREGNERRWKR 582
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