Conserved Protein Domain Family
fadh2_euk

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TIGR00677: fadh2_euk 
methylenetetrahydrofolate reductase, eukaryotic type
The enzyme activities methylenetetrahydrofolate reductase (EC 1.5.1.20) and 5,10-methylenetetrahydrofolate reductase (FADH) (EC 1.7.99.5) differ in that 1.5.1.20 (assigned in many eukaryotes) is defined to use NADP+ as an acceptor, while 1.7.99.5 (assigned in many bacteria) is flexible with respect to the acceptor; both convert 5-methyltetrahydrofolate to 5,10-methylenetetrahydrofolate. From a larger set of proteins assigned as 1.5.1.20 and 1.7.99.5, this model describes the subset of proteins found in eukaryotes and designated 1.5.1.20. This protein is an FAD-containing flavoprotein. [Biosynthesis of cofactors, prosthetic groups, and carriers, Folic acid]
Statistics
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PSSM-Id: 129760
View PSSM: TIGR00677
Aligned: 8 rows
Threshold Bit Score: 479.614
Threshold Setting Gi: 1709159
Created: 7-Oct-2014
Updated: 23-Jan-2015
Structure
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Aligned Rows:
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5911425   17 AFSFEFFPPKTEDGVENLFERMDRL-VSY-GPTFCDITW----GAGGSTADL-TLEIASRMQNVI-CVETMMHLTCTNMP 88
gi 5802606   17 AFSFEYFPPKTEEGVENLFERMDRM-VAH-GPSFCDITW----GAGGSTADL-TLEIANRMQNMV-CVETMMHLTCTNMP 88
gi 1723442   17 TYSYEFFPPKTSTGVQNLYNRIDRM-KTWgRPMFVDVTW----GAGGTSSEL-TPGIVNVIQTDF-EVDTCMHLTCTNMS 89
gi 1628449   18 TYSFEYFVPKTTQGVQNLYDRMDRMyEAS-LPQFIDITW----NAGGGRLSH-LSTDLVATRQSVlGLETCMHLTCTNMP 91
gi 1709159   14 FISLEFFPPKTELGTRNLMERMHRM-TAL-DPLFITVTW----GAGGTTAEK-TLTLASLAQQTL-NIPVCMHLTCTNTE 85
gi 18406468  17 AFSFEFFPPKTEDGVDNLFERMDRM-VAY-GPTFCDITW----GAGGSTADL-TLDIASRMQNVV-CVESMMHLTCTNMP 88
gi 1322687   18 TYSFEYFVPKTTQGVQNLYDRMDRMyEAS-LPQFIDITW----NAGGGRLSHlSTDLVATAQSVL-GLETCMHLTCTNMP 91
gi 4336819   59 WFSLEFFPPRTAEGAVNLISRFDRM-AAG-GPLYIDVTWhpagDPGSDKETS-SMMIASTAVNYC-GLETILHMTCCRQR 134
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5911425   89 IEKIDHALETIRSNGIQNVLALRGDPPHGQDKF---VQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVieadg 165
gi 5802606   89 VEKIDHALETIKSNGIQNVLALRGDPPHGQDKF---VQVEGGFACALDLVQHIRAKYGDYFGITVAGYPEAHPDA----- 160
gi 1723442   90 TEMIDAALKRAHETGCRNILALRGD-PVKDTDW---TEGESGFRYASDLVRYIRTHYNDEFCIGVAGYPEGYSPD----- 160
gi 1628449   92 ISMIDDALENAYHSGCQNILALRGDPPRDAENW---TPVEGGFQYAKDLIKYIKSKYGDHFAIGVAGYPECHPEL----- 163
gi 1709159   86 KAIIDDALDRCYNAGIRNILALRGD-PPIGEDWldsQSNESPFKYAVDLVRYIKQSYGDKFCVGVAAYPEGHCEG----- 159
gi 18406468  89 VEKIDHALETIRSNGIQNVLALRGDPPHGQDKF---VQVEGGFDCALDLVNHIRSKYGDYFGITVAGYPEAHPDVigeng 165
gi 1322687   92 ISMIDDALENAYHSGCQNILALRGDPPRDAENW---TPVEGGFQYAKDLIKYIKSKYGDHFAIGVAGYPECHPEL----- 163
gi 4336819  135 LEEITGHLHKAKQLGLKNIMALRGDP--IGDQW---EEEEGGFNYAVDLVKHIRSEFGDYFDICVAGYPKGHPEA----- 204
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5911425  166 latpES--Y------QS---------DLAYLKKKVDAGADLIVTQLFYDTDIFLKFVNDCRQ-IGINCPIVPGIMPISNY 227
gi 5802606  161 ----IQ--G------EGgatleaysnDLAYLKRKVDAGADLIVTQLFYDTDIFLKFVNDCRQ-IGITCPIVPGIMPINNY 227
gi 1723442  161 ----DD--I------DE---------SIKHLKLKVDEGADFIVTQMFYDVDNFIAWVDKVRA-AGINIPIFPGIMPIQAW 218
gi 1628449  164 ----PNkdV------KL---------DLEYLSRR-STGGDFIITQMFYDVDNLLNWCSQVRA-AGMDVPIIPGIMPITTY 222
gi 1709159  160 ----EA--EgheqdpLK---------DLVYLKEKVEAGADFVITQLFYDVEKFLTFEMLFRErISQDLPLFPGLMPINSY 224
gi 18406468 166 lasnEA--Y------QS---------DLEYLKKKIDAGADLIVTQLFYDTDIFLKFVNDCRQ-IGISCPIVPGIMPINNY 227
gi 1322687  164 ----PNkdV------KL---------DLEYLSRR-STGGDFIITQMFYDVDNLLNWCSQVRA-AGMDVPIIPGIMPITTY 222
gi 4336819  205 ----GS--F------EA---------DLKHLKEKVSAGADFIITQLFFEADTFFRFVKACTD-MGITCPIVPGIFPIQGY 262
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5911425  228 KGFLRMAGFCKTKIPAELTAAL-EPIKDNDEAVKAYGIHFATEMCKKILAH---G-ITSLHLYTLNVDKSAIGILMNLGL 302
gi 5802606  228 KGFLRMTGFCKTKIPSEITAAL-DPIKDNEEAVRQYGIHLGTEMCKKILAT---G-IKTLHLYTLNMDKSAIGILMNLGL 302
gi 1723442  219 DSFIRRAKWSGVKIPQHFMDTL-VPVKDDDEGVRERGVELIVEMCRKLIAS---G-ITRLHFYTMNLEKAVKMIIERLGL 293
gi 1628449  223 AAFLRRIQWGQISIPQHFSSRL-DPIKDDDELVRDIGTNLIVEMCQKLLDS---GyVSHLHIYTMNLEKAPLMILERLNI 298
gi 1709159  225 LLFHRAAKLSHASIPPAILSRFpPEIQSDDNAVKSIGVDILIELIQEIYQRtsgR-IKGFHFYTLNLEKAIAQIVSQSPV 303
gi 18406468 228 RGFLRMTGFCKTKIPVEVMAAL-EPIKDNEEAVKAYGIHLGTEMCKKMLAH---G-VKSLHLYTLNMEKSALAILMNLGM 302
gi 1322687  223 AAFLRRIQWGQISIPQHFSSRL-DPIKDDDELVRDIGTNLIVEMCQKLLDS---GyVSHLHIYTMNLEKAPLMILERLNI 298
gi 4336819  263 HSLRQLVKLSKLEVPQEIKDVI-EPIKDNDAAIRNYGIELAVSLCQELLAS---GlVPGLHFYTLNREMATTEVLKRLGM 338

                ....
gi 5911425  303 IDES 306
gi 5802606  303 IEES 306
gi 1723442  294 LDEN 297
gi 1628449  299 LPTE 302
gi 1709159  304 LSHI 307
gi 18406468 303 IDES 306
gi 1322687  299 LPTE 302
gi 4336819  339 WTED 342
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