Conserved Protein Domain Family
nst

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TIGR00803: nst 
UDP-galactose transporter
The 10-12 TMS Nucleotide Sugar Transporters (TC 2.A.7.10)Nucleotide-sugar transporters (NSTs) are found in the Golgi apparatus and the endoplasmic reticulum of eukaryotic cells. Members of the family have been sequenced from yeast, protozoans and animals. Animals such as C. elegans possess many of these transporters. Humans have at least two closely related isoforms of the UDP-galactose:UMP exchange transporter.NSTs generally appear to function by antiport mechanisms, exchanging a nucleotide-sugar for a nucleotide. Thus, CMP-sialic acid is exchanged for CMP; GDP-mannose is preferentially exchanged for GMP, and UDP-galactose and UDP-N-acetylglucosamine are exchanged for UMP (or possibly UDP). Other nucleotide sugars (e.g., GDP-fucose, UDP-xylose, UDP-glucose, UDP-N-acetylgalactosamine, etc.) may also be transported in exchange for various nucleotides, but their transporters have not been molecularly characterized. Each compound appears to be translocated by its own transport protein. Transport allows the compound, synthesized in the cytoplasm, to be exported to the lumen of the Golgi apparatus or the endoplasmic reticulum where it is used for the synthesis of glycoproteins and glycolipids.
Statistics
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PSSM-Id: 129885
View PSSM: TIGR00803
Aligned: 10 rows
Threshold Bit Score: 183.71
Threshold Setting Gi: 1041825
Created: 8-Oct-2014
Updated: 23-Jan-2015
Structure
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Aligned Rows:
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 731393     94 NISIPIHIVFRCFGTVITMFTCWLLNGRKYT-KIQILSTLFLTIGAIIASLFKDADFRYQDLKLQAWKIGSDQSVD---- 168
gi 7770097   114 KLAVPSLIYTLQNNLQYVAISNLPAATFQVTyQLKILTTALFSVLMLNRSLSRLQWASLLLLFTGVAIVQAQQAGGgg-p 192
gi 3298605    86 KLAIPSGIYTLQNNLLYVALSNLDAATYQVTyQLKILTTALFSVSMLSKKLGVYQWLSLVILMTGVAFVQWPSDSQelds 165
gi 12644363   97 KLAIPAFLYTCQNNLQYVAAGNLTAASFQVTyQLKILTTAIFSILLLHRRLGPMKWFSLFLLTGGIAIVQLQNLNSd--- 173
gi 2499225    90 KLSVPSLVYAVQNNMAFLALSNLDAAVYQVTyQLKIPCTALCTVLMLNRTLSKLQWVSVFMLCGGVILVQWKPAQAt--- 166
gi 729611    108 yLAVPIYTIFKNLTIILIAYGEVLFFGGSVT-SMELSSFLLMVLSSVVATWGDQQAVAAKAASLAEGAAGAVAS------ 180
gi 189047106 103 yLAVPIYTIFKNLTIILIAYGEVLFFGGKVT-SMELTSFIMMVLSSVVATWGDQQAIAIKASSLEDLDQELVESTI---- 177
gi 1041825    97 TMSVAAQTILKNLAVVLIALGDKFLYGKAQT-PMVYFSFALMILGSLLGAKGDKWVTAWGLVWTFLNIVSTVSYT----- 170
gi 1373152    93 DISVPIHIIIRCSGTTLTMIIGWAVCNKRYS-KLQVQSAIIMTLGAIVASLYRDKEFSMDSLKLNTDSVGMTQKS----- 166
gi 17556923  122 KMSVPSFAYALQNNLDFVGLSNLDAGLYQVTtQLKVVSTAFFMMLFLGRKFSTRRWMAITLLMFGVAFVQMNNVSAse-a 200
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 731393    169 LTFIFg--ICILVLSSFTSSLLSAYNERTYQKYGKHWKENI-FYSHFLSLPLFLFSRKqliheYRVMRKSERILCSNFGG 245
gi 7770097   193 RPLDQ---NPGAGLAAVVASCLSSGFAGVYFEKILKGSSGS-VWLRNLQLGLFGTALG-----LVGLWWAEGTAVATRGF 263
gi 3298605   166 KELSAg--SQFVGLMAVLTACFSSGFAGVYFEKILKETKQS-VWIRNIQLGFFGSIFG-----LMGVYIYDGELVSKNGF 237
gi 12644363  174 DQMSAgpmNPVTGFSAVLVACLISGLAGVYFEKVLKDTNPS-LWVRNVQLSFFSLFPC-----LFTILMKDYHNIAENGF 247
gi 2499225   167 KVVVEq--SPLLGFGAIAIAVLCSGFAGVYFEKVLKSSDTS-LWVRNIQMYLSGIVVT-----LVGTYLSDGAEIKEKGF 238
gi 729611    181 --FNPg--YFWMFTNCITSALFVLIMRKRIKLTNFKDFDTM-FYNNVLALPILLLFS------FCVEDWSSVNLTNNFS- 248
gi 189047106 178 FVLNPg--YLWMFTNCISSALFVLIMRKRIRLTNFKDYDTM-FYNNVLALPLLLVFS------FIMEDWSTKNLSVNLS- 247
gi 1041825   171 -LYMKavlGSVSNSIGRYGPVFYNNLLSLPFFLIMGVGDIMpFSAAIGDTTTFGKLVLt----FSVLVSSVMTFSVFWCM 245
gi 1373152   167 ---MFg--IFVVLVATALMSLLSLLNEWTYNKCGKHWKETL-FYSHFLALPLFMLGYTrlrdeFRDLLISSDSMDIPIV- 239
gi 17556923  201 NTKREtaeNYIVGLSAVLATCVTAGFAGVYFEKMLKDGGSTpFWIRNMQMYSCGVIS------ASIACLTDFSRISDKGF 274
                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 731393    246 KILVPREETLLLFNVLTQYFCVKGVNILASKTnalTLSITLLVRKFISLLLS-VRLFDNNLSYTGYIGVYLVFFGAFIY 323
gi 7770097   264 FFGYTPAVWGVVLNQAFGGLLVAVVVKYADNI---LKGFATSLSIVLSTVAS-IRLFGFHVDPLFALGAGLVIGAVYLY 338
gi 3298605   238 FQGYNRLTWIVVILQALGGLVIAAVIKYADNI---LKGFATSLSIILSTLISyFWLQDFVPTSVFFLGAILVITATFLY 313
gi 12644363  248 FFGYNSIVWLAILLQAGGGIIVALCVAFADNI---MKNFSTSISIIISSLAS-VYLMDFKISLTFLIGVMLVIAATFLY 322
gi 2499225   239 FYGYTYYVWFVIFLASVGGLYTSVVVKYTDNI---MKGFSAAAAIVLSTIAS-VMLFGLQITLSFAMGALLVCISIYLY 313
gi 729611    249 -NDSLTAMIISGVASVGISYCSGWCVRVTSST---TYSMVGALNKLPIALSG-LIFFDAPRNFLSILSIFIGFLSGIIY 322
gi 189047106 248 -ADSLAAMVISGLMSVGISYCSGWCVRVTSST---TYSMVGALNKLPIALAG-LVFFDAPKNFLSFFSIFLGFLSGLLY 321
gi 1041825   246 SITSPTTMSVVGSLNKIPLTFLGMLVFHQFPTatgYLGIMIALSAGFLYTHLnIRANRAKASSDTEHQMQQAGKTTAES 324
gi 1373152   240 KLPIATKLFMLIANNVTQFICIKGVNMLASNTdalTLSVVLLVRKFVSLLLS-VYIYKNVLSVTAYLGTITVFLGAGLY 317
gi 17556923  275 FFGYTDKVWAVVILLGVGGLYISLVMRYLDNL---YKSMASAVSIILVVVLSmLIFPDIFIGMYFVLGTICVVLAVLLY 350
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