Conserved Protein Domain Family
tpt

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TIGR00817: tpt 
Tpt phosphate/phosphoenolpyruvate translocator
The 6-8 TMS Triose-phosphate Transporter (TPT) Family (TC 2.A.7.9)Functionally characterized members of the TPT family are derived from the inner envelope membranes of chloroplasts and nongreen plastids of plants. However,homologues are also present in yeast. Saccharomyces cerevisiae has three functionally uncharacterized TPT paralogues encoded within its genome. Under normal physiologicalconditions, chloroplast TPTs mediate a strict antiport of substrates, frequently exchanging an organic three carbon compound phosphate ester for inorganic phosphate (Pi).Normally, a triose-phosphate, 3-phosphoglycerate, or another phosphorylated C3 compound made in the chloroplast during photosynthesis, exits the organelle into thecytoplasm of the plant cell in exchange for Pi. However, experiments with reconstituted translocator in artificial membranes indicate that transport can also occur by achannel-like uniport mechanism with up to 10-fold higher transport rates. Channel opening may be induced by a membrane potential of large magnitude and/or by high substrateconcentrations. Nongreen plastid and chloroplast carriers, such as those from maize endosperm and root membranes, mediate transport of C3 compounds phosphorylated atcarbon atom 2, particularly phosphenolpyruvate, in exchange for Pi. These are the phosphoenolpyruvate:Pi antiporters (PPT). Glucose-6-P has also been shown to be asubstrate of some plastid translocators (GPT). The three types of proteins (TPT, PPT and GPT) are divergent in sequence as well as substrate specificity, but their substratespecificities overlap. [Hypothetical proteins, Conserved]
Statistics
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PSSM-Id: 129898
View PSSM: TIGR00817
Aligned: 10 rows
Threshold Bit Score: 297.793
Threshold Setting Gi: 731957
Created: 8-Oct-2014
Updated: 23-Jan-2015
Structure
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Aligned Rows:
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1711397 112 ALTELQFLVSAVLCVGFAsIVNlFRLPRLKHTKFSKALNSFPDGILPEYLDgnfRSSILHKFLVPSKLVLMTTFPMGIFQ 191
gi 1778143 108 LVLGSLFGLWYIFNIYFN-IYN-KQVLKTFHYPVTITLAQLAVGTILVIFM---WTSNLYKRPKISGAQLAAILPLAVVH 182
gi 1706110 100 LELGLLFAMWYLFNIYFN-IYN-KQVLKALHAPMTVTLVQFAVGSVLITFM---WALNLYKRPKISAAQLAAILPLAVVH 174
gi 1778149  94 LQLGSLFGLWYLFNIYFN-IYN-KQVLKVLPYPINITTVQFAVGSAIALFM---WITGILKRPKISGAQLFAILPLAIVH 168
gi 3367515  95 LKIGIYFATWWALNVVFN-IYN-KKVLNAFPYPWLTSTLSLACGSLMMLVS---WATRIADAPKTDLEFWKTLFPVAVAH 169
gi 3983125 106 LVTGFFFFMWYFLNVIFN-ILN-KKIYNYFPYPYFVSVIHLFVGVVYCLIS---WSVGLPKRAPIDSNLLKVLIPVAVCH 180
gi 117291  100 LVTGSFFFMWYFLNVIFN-ILN-KKIYNYFPYPYFVSVIHLFVGVVYCLAS---WSVGLPKRAPMDSKLLKLLIPVAVCH 174
gi 231903  110 LTTGFFFFMWYFLNVIFN-ILN-KKIYNYFPYPYFVSVIHLAVGVVYCLVS---WGVGLPKRAPIDSTQLKLLTPVAFCH 184
gi 4488    111 ALTELQFLVSAVLCVGFAsIVNlFRLPRLKHTKFSKALNSFPDGILPEYLDgnfRSSILHKFLVPSKLVLMTTFPMGIFQ 190
gi 731957  112 LQTVLPLGLFQFVGKYFG--HTaTSLVPVSTVASIKTLSPMFILLLQKILK----ISTLKITLTLIFSLCTLVLGVWIIV 185
                       90       100       110       120       130       140       150       160
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1711397 192 FIGHITSHKAVSMIPVSLVHSVKALSPIITVGYYKFFEHrYYNsMTYY--TLLLLIFGVMttcwsthgskRASDNKSGSS 269
gi 1778143 183 TLGNLFTNMSLGKVSVSFTHTIKAMEPFFSVVLSAMFLG-EFP-TLWVisSLVPIVGGVG----------LASLTEASFN 250
gi 1706110 175 TLGNLFTNMSLGKVSVSFTHTIKAMEPFFSVVLSAMFLG-EVP-TPWVigSIIPIVGGVA----------LASVTEVSFN 242
gi 1778149 169 TMGNLFTNMSLGKVAVSFTHTIKAMEPFFSVLLSAIFLG-ELP-TPWVvlSLLPIVGGVA----------LASLTEASFN 236
gi 3367515 170 TIGHVAATVSMSKVAVSFTHIIKSGEPAFSVLVSRFFMGeTFPlPVYL--SLLPIIGGCA----------LAAITELNFN 237
gi 3983125 181 ALGHVTSNVSFAAVAVSFTHTIKALEPFFNAAASQFIMGqSIPiTLWL--SLAPVVLGVA----------MASLTELSFN 248
gi 117291  175 AIGHVTSNVSFAAVAVSFTHTIKALEPFFNAAASQFVLGqSIPiTLWL--SLAPVVIGVS----------MASLTELSFN 242
gi 231903  185 ALGHVTSNVSFAAVRVSFTHTVKALEPFFNAAASQFILGqQIPlALWL--SLAPVVLGVS----------MASLTELSFN 252
gi 4488    191 FIGHITSHKAVSMIPVSLVHSVKALSPIITVGYYKFFEHrYYNsMTYY--TLLLLIFGVMttcwsthgskRASDNKSGSS 268
gi 731957  186 QEDNRSPASSNELREFSKYGVICAMISMFIFVLQNIYGKtVFT---------YR----------------SQTDESQSNS 240
                      170       180       190       200       210       220       230       240
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1711397 270 LIGLLFAFISMIIFVAQNIFAKNILTIRrkvgilpssstddvtskegqpSLDKTRFSPLQVdkITILFYCSCIGFSLTLL 349
gi 1778143 251 WAGFWSAMACNLTNQSRNVLSKKFMVRKe-------------------eSLDNITLFSIIT--IMSFILLAPFAFFMEGV 309
gi 1706110 243 WAGFLSAMASNLTNQSRNVLSKKVMVKKd-------------------dSLDNITLFSIIT--LMSLFLMAPVTFFSEGI 301
gi 1778149 237 WAGFWSAMASNVTFQSRNVLSKKLMVKKe-------------------eSLDNINLFSIIT--VMSFFLLAPVTLLTEGV 295
gi 3367515 238 ITGFMGAMISNLAFVFRNIFSKKGMKGK---------------------SVSGMNYYACLS--MMSLVILTPFSIAVEGP 294
gi 3983125 249 WLGFISAMISNISFTYRSIFSKKAMT-----------------------DMDSTNVYAYIS--IIALFVCIPPAIIVEGP 303
gi 117291  243 WLGFISAMISNVSFTYRSLYSKKAMT-----------------------DMDSTNIYAYIS--IIALFVCLPPAIIVEGP 297
gi 231903  253 WLGFTSAMISNISFTYRSIYSKKAMT-----------------------DMDSTNVYAYIS--IIALIFCLPPAIFIEGP 307
gi 4488    269 LIGLLFAFISMIIFVAQNIFAKNILTIRrkvgilpssstddvtskegqpSLDKTRFSPLQVdkITILFYSSCIGFSLTLL 348
gi 731957  241 GFSRQESPLPLYEKLDEKLVAKKKPK-----------------------SYDKLTLMIYIS---LVGFCLSFGWFITLEF 294
                      250       260       270       280       290       300       310       320
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1711397 350 PFLTgELMHGgSVINDLTLETVALVAIHgIAHFFQaMLAFQLIGLLSSINYSVANIMKRIVVISV-ALFWETKLNFFQVF 428
gi 1778143 310 KFTP-AYLEA-SGLNVNQIYTRSLLAAL-CFHAYQ-QVSYMILERVSPVTHSVGNCVKRVVVIVTsVLFFRTPVSPINTI 385
gi 1706110 302 KFTP-SYIQS-AGVNVQQIYTKSLIAAL-CFHAYQ-QVSYMILARVSPVTHSVGNCVKRVVVIVSsVIFFKTPVSPVNAF 377
gi 1778149 296 KVSP-AVLQS-AGLNLKQIYTRSLIAAC-CFHAYQ-QVSYMILARVSPVTHSVGNCVKRVVVIVTsVLFFRTPVSPINSL 371
gi 3367515 295 QMWAaGWQNAvSQVGPNFVW--WVVAQSvFYHLYN-QVSYMSLDQISPLTFSIGNTMKRISVIVAsIIIFHTPIQPVNAL 371
gi 3983125 304 KLLNhGFADAiAKVGMTKFISDLFWVGM-FYHLYN-QLATNTLERVAPLTHAVGNVLKRVFVIGFsIVIFGNKISTQTGI 381
gi 117291  298 QLMKhGFNDAiAKVGLTKFISDLFWVGM-FYHLYN-QLATNTLERVAPLTHAVGNVLKRVFVIGFsIIAFGNKISTQTAI 375
gi 231903  308 QLLQhGFNDAiAKVGLTKFVTDLFWVGM-FYHLYN-QVATNTLERVAPLTHAVGNVLKRVFVIGFsIVIFGNKISTQTGI 385
gi 4488    349 PFLTgELMHGgSVINDLTLETVALVAIHgIAHFFQaMLAFQLIGLLSSINYSVANIMKRIVVISV-ALFWETKLNFFQVF 427
gi 731957  295 PVLFrYFFQInSSSTVIKAFPVSLFLLNgTFHFIQaMITFHLLGEVSTLTYSIANLMKRFAIIAVsWVFIGRRITWLQVF 374
                      330       340
               ....*....|....*....|....*..
gi 1711397 429 GVILTIAGLYGYDKWGLS----KKDGR 451
gi 1778143 386 GTGVALAGVFLYSRVKGI----KPKPK 408
gi 1706110 378 GTGIALAGVFLYSRVKRI----KPKPK 400
gi 1778149 372 GTGIALAGVFLYSQLKRL----KPKPK 394
gi 3367515 372 GAAIAIFGTFLYSQVNKQtyscLPTSS 398
gi 3983125 382 GTGIAIAGVAMYSIIKAK----IEEEK 404
gi 117291  376 GTSIAIAGVALYSLIKAK----MEEEK 398
gi 231903  386 GTCIAIAGVAIYSFIKAK----MEEEK 408
gi 4488    428 GVILTIAGLYGYDKWGLS----KKDGR 450
gi 731957  375 GLVLNTLGLFLYERCTSQs---KIKAK 398
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