Conserved Protein Domain Family
PNK-3'Pase

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TIGR01663: PNK-3'Pase 
polynucleotide 5'-kinase 3'-phosphatase
This model represents the metazoan 5'-polynucleotide-kinase-3'-phosphatase, PNKP, which is believed to be involved in repair of oxidative DNA damage. Removal of 3' phosphates is essential for the further processing of the break by DNA polymerases. The central phosphatase domain is a member of the IIIA subfamily (TIGR01662) of the haloacid dehalogenase (HAD) superfamily of aspartate-nucleophile hydrolases. As is common in this superfamily, the enzyme is magnesium dependent. A difference between this enzyme and other HAD-superfamily phosphatases is in the third conserved catalytic motif which usually contains two conserved aspartate residues believed to be involved in binding the magnesium ion. Here, the second aspartate is replaced by a conserved arginine residue which may indicate an interaction with the phosphate backbone of the substrate. Very close relatives of this domain are also found separate from the N- and C-terminal domains seen here, as in the 3'-phosphatase found in plants. The larger family of these domains is described by TIGR01664. Outside of the phosphatase domain is a P-loop ATP-binding motif associated with the kinase activity. The entry for the mouse homolog appears to be missing a large piece of sequence corresponding to the first conserved catalytic motif of the phosphatase domain as well as the conserved threonine of the second motif. Either this is a sequencing artifact or this may represent a pseudo- or non-functional gene. Note that the EC number for the kinase function is: 2.7.1.78
Statistics
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PSSM-Id: 130724
View PSSM: TIGR01663
Aligned: 2 rows
Threshold Bit Score: 983.362
Threshold Setting Gi: 7299027
Created: 8-Oct-2014
Updated: 23-Jan-2015
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 14211141   2 GEVEAPGRLWLES----PPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQVELVADPETRTVAVKQLGVNPSTTGTQ 77
gi 7299027    7 RSVGKAGKDVAARictlKPTEPEHHSIHLTAGENFVGRSRETGIRDSKCSKRQIQLQVDLKKAVVSLKVLGVNPCGVNGL 86
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 14211141  78 ELKPGLEGSLGVGDTLYLVNGLHpltlRWEETRTPESQPDTPPGTPLVSQDEkrDAELPKKRMRKSNpgwenlEKLLVFT 157
gi 7299027   87 MLMQNGERELKHGDLVEIVYGRH----QFEVVFNPPPEYDKEKAEPLSTTLS--PSEKSERWDSSGN------GKLVIFT 154
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 14211141 158 AAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVE 237
gi 7299027  155 SVGVKGSEKIAGYDMDGTIIKTKSGLVFPKNTDDWQIIFPEVPEKLKNLHKDGFKICLFTNQGGIARGKINLDDFKVKIK 234
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 14211141 238 AVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPAN-WAPGRKKKDFSCADRLFALN 316
gi 7299027  235 HIVAKLGVPIQVFIAIGDGFYRKPLTGMWQHLKSEMNDGVEIQEDRCFFVGDAAGRPETgKGATKRRKDHSLVDRLFAAN 314
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 14211141 317 LGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNR 396
gi 7299027  315 VGISFYTPEVHFLGKQVEQWNKPDFEPTSVQDQVSLLDPDDLTLDDHPCEMVIMVGLPGSGKSHFCSSFFQPRGYKIVNA 394
                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 14211141 397 DTLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSD 476
gi 7299027  395 DTLGSTQNCLTACKRFLDSGQSCVVDNTNVDAASRKKFLQLASEKMIPCRCLVMNVPVAQVKHNIAFRELSDSARTKIKD 474
                       490       500       510       520
                ....*....|....*....|....*....|....*....|....*.
gi 14211141 477 MVMYGYRKQFEAPTLAEGFSAILEIPFR-LWVEPRLGRLYCQFSEG 521
gi 7299027  475 MVFNMMKKKYQEPALDEGFISIHKVNFKpNFADEKQEKLYKMYLVE 520
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