1GUZ,1I0Z,1Y6J,1LDN,1HYH,1EZ4


Conserved Protein Domain Family
LDH_like

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cd00300: LDH_like 
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L-lactate dehydrogenase-like enzymes
Members of this subfamily are tetrameric NAD-dependent 2-hydroxycarboxylate dehydrogenases including LDHs, L-2-hydroxyisocaproate dehydrogenases (L-HicDH), and LDH-like malate dehydrogenases (MDH). Dehydrogenases catalyze the conversion of carbonyl compounds to alcohols or amino acids. LDHs catalyze the last step of glycolysis in which pyruvate is converted to L-lactate. Vertebrate LDHs are non-allosteric, but some bacterial LDHs are activated by an allosteric effector such as fructose-1,6-bisphosphate. L-HicDH catalyzes the conversion of a variety of 2-oxo carboxylic acids with medium-sized aliphatic or aromatic side chains. MDH is one of the key enzymes in the citric acid cycle, facilitating both the conversion of malate to oxaloacetate and replenishing levels of oxalacetate by reductive carboxylation of pyruvate. The LDH-like subfamily is part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
Statistics
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PSSM-Id: 133418
View PSSM: cd00300
Aligned: 15 rows
Threshold Bit Score: 268.368
Threshold Setting Gi: 29826879
Created: 30-Apr-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              ## #                    # #                                          #####
1GUZ_A         3 ITVIGAGN-VGATTAFRLAEkqlarELVLLDVVegIPQGKALDMYESGpvg-lfDTKVTGSNdYADTANSDIVIITAGLP 80
gi 32472038    3 VSIIGAGGlVGSCAAYALQCgglarEIALLDVNveTAVGHALDLQHGSps--vaDQTIVGGG-YEHIPDSDVICITAGLR 79
gi 182413118   3 LTIIGGGGrVGSNAAFALQCagivsEIQLLDANaeLAAGEALDLLHGSas--vaDQRIYAGD-YARAADSDMFVVTAGLR 79
gi 49035986    7 IVVIGASN-VGSAVANKIADfqlatEVVLIDLNedKAWGEAKDSSHATsciystNIKFHLGD-YEDCKDANIIVITAGPS 84
gi 32471115    3 ITLVGTGR-VGSAIAFALTInplasELLLLNRSreKAEGDALDLTHAAalv-dsNIKISSGE-IADSKDSDVIIFTASVP 79
1I0Z_A        23 ITVVGVGQ-VGMACAISILGksladELALVDVLedKLKGEMMDLQHGSlf--lqTPKIVADKdYSVTANSKIVVVTAGVR 99
1Y6J_A        10 VAIIGAGF-VGASAAFTMALrqtanELVLIDVFkeKAIGEAMDINHGLpf---mGQMSLYAGdYSDVKDCDVIVVTAGAN 85
1LDN_A         9 VVVIGAGF-VGASYVFALMNqgiadEIVLIDANesKAIGDAMDFNHGKvfa-pkPVDIWHGD-YDDCRDADLVVICAGAN 85
1HYH_C         4 IGIIGLGN-VGAAVAHGLIAqgvadDYVFIDANeaKVKADQIDFQDAManl-eaHGNIVIND-WAALADADVVISTLGNI 80
1EZ4_A         8 VVLVGDGA-VGSSYAFAMAQqgiaeEFVIVDVVkdRTKGDALDLEDAQaf--taPKKIYSGE-YSDCKDADLVVITAGAP 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                   # #                      #   #       
1GUZ_A        81 RK-PGMTRe----dLLMKNAGIVKEVTDNIMKhs--kNPIIIVVSNPLDIMTHVAwvrsglpkERVIGMAgVLDAARFRS 153
gi 32472038   80 RK-PDESRl----dLINRNTDLFVQILRDVKAagpkqSAIVLVVSNPVDILTYVAagmlglpiKQVIGLGtQLDTIRFCS 154
gi 182413118  80 RK-PDESRl----dLINRNVALFDSILASIKQaglrkDALVFVVSNPVDTLTQLAvarlglpsQQVLGLGtMLDTARFRS 154
gi 49035986   85 IR-PGETPdr--lkLAGTNAKIMSSVMGEIVKrt--kEAMIIMITNPLDVATYVVstqfdyprNLILGTGtMLETYRFRR 159
gi 32471115   80 FRyPNQTRl----eMGIDNMPILRDWMPGLAKas--pNAIVVMVSNPVDALAYETirltgfdpKRVIGTGtLVDSIRYRA 153
1I0Z_A       100 QQ-EGESRl----nLVQRNVNVFKFIIPQIVKys--pDCIIIVVSNPVDILTYVTwklsglpkHRVIGSGcNLDSARFRY 172
1Y6J_A        86 RK-PGETRl----dLAKKNVMIAKEVTQNIMKyy--nHGVILVVSNPVDIITYMIqkwsglpvGKVIGSGtVLDSIRFRY 158
1LDN_A        86 QK-PGETRl----dLVDKNIAIFRSIVESVMAsg--fQGLFLVATNPVDILTYATwkfsglphERVIGSGtILDTARFRF 158
1HYH_C        81 KL-QQDNPtgdrfaELKFTSSMVQSVGTNLKEsg--fHGVLVVISNPVDVITALFqhvtgfpaHKVIGTGtLLDTARMQR 157
1EZ4_A        84 QK-PGESRl----dLVNKNLNILSSIVKPVVDsg--fDGIFLVAANPVDILTYATwkfsgfpkERVIGSGtSLDSSRLRV 156
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                            #                                                        #  
1GUZ_A       154 FIAMELGvsmqDINACVLGGHGDAMVPVVKYTTVAGIpisdll-------paetiDKLVERTRNGGAEIVEhlkqGSAFY 226
gi 32472038  155 LIAEELKapptQTKALILGEHGDTMVPIWSSAAIGSLplekfpg-----wtpalaNQLFTRTRGSGAEVIKr--kGGAGF 227
gi 182413118 155 LIAEQLKlaptQVKALILGEHGDTMVPIWSSATAAGLplekmpg-----vtpsfqQQIFERTKTSGAEVIRr--kGGAGW 227
gi 49035986  160 ILADKYQvdpkNINGYVLGEHGNAAFVAWSTTGCAGFpiddldeyf-hrteklshEAVEQELVQVAYDVINk--kGFTNT 236
gi 32471115  154 LLSTELKihaqDIRAYILGEHGDTQFAASSIAMTGGErfyp----------sdtsRRMFEETKAMGYEVFRl--kGHTSY 221
1I0Z_A       173 LMAEKLGihpsSCHGWILGEHGDSSVAVWSGVNVAGVslqelnpemgtdndsenwKEVHKMVVESAYEVIKl--kGYTNW 250
1Y6J_A       159 LLSEKLGvdvkNVHGYIIGEHGDSQLPLWSCTHIAGKnineyiddpkcnfteedkKKIAEDVKTAGATIIKn--kGATYY 236
1LDN_A       159 LLGEYFSvapqNVHAYIIGEHGDTELPVWSQAYIGVMpirklvesk-geeaqkdlERIFVNVRDAAYQIIEk--kGATYY 235
1HYH_C       158 AVGEAFDldprSVSGYNLGEHGNSQFVAWSTVRVMGQpivtlad-----agdidlAAIEEEARKGGFTVLNg--kGYTSY 230
1EZ4_A       157 ALGKQFNvdprSVDAYIMGEHGDSEFAAYSTATIGTRpvrdvake--qgvsdddlAKLEDGVRNKAYDIINl--kGATFY 232
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
Feature 1         #                                                                            
1GUZ_A       227 APASSVVEMVESIVLdRKRVLPCAVGLEgqyg-idkTFVGVPVKLGRNGVEqIYEINLDqADLDLLQKSAKIVDENCK 303
gi 32472038  228 AVGIAIRDVIDAVILdRKCLLPVSSVQSgcyg-ihdVALSVPTVVGRAGVVdQLEIDLWpKEVQGLRASGAALRKTLN 304
gi 182413118 228 AVAITIAEVVHAIAQdKRQLLPVSSLQQgcyg-lrdVALSVPTVVGRGGVIsHVEIPLWpKEQQGLQASARALQEIWS 304
gi 49035986  237 GIAMAACRFIKSVLYdEHTILPCSAVLEgeyg-ikdVALSIPRMVCADGIMrSFEVHLTdDELEKMHKAAQSVRSALD 313
gi 32471115  222 GIAMATITILDSIAYdLRHTMPVSVLVDgylg-vedVCLSLPAVIGREGVTrILHPTLSeDEEAAFRNSAEVVKQTLV 298
1I0Z_A       251 AIGLSVADLIESMLKnLSRIHPVSTMVKgmygieneVFLSLPCILNARGLTsVINQKLKdDEVAQLKKSADTLWDIQK 328
1Y6J_A       237 GIAVSINTIVETLLKnQNTIRTVGTVINgmyg-iedVAISLPSIVNSEGVQeVLQFNLTpEEEEALRFSAEQVKKVLN 313
1LDN_A       236 GIAMGLARVTRAILHnENAILTVSAYLDglyg-erdVYIGVPAVINRNGIReVIEIELNdDEKNRFHHSAATLKSVLA 312
1HYH_C       231 GVATSAIRIAKAVMAdAHAELVVSNRRDdm-----gMYLSYPAIIGRDGVLaETTLDLTtDEQEKLLQSRDYIQQRFD 303
1EZ4_A       233 GIGTALMRISKAILRdENAVLPVGAYMDgqyg-lndIYIGTPAIIGGTGLKqIIESPLSaDELKKMQDSAATLKKVLN 309

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