Conserved Protein Domain Family
Ribosomal_L7_L12

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cd00387: Ribosomal_L7_L12 
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Ribosomal protein L7/L12. Ribosomal protein L7/L12 refers to the large ribosomal subunit proteins L7 and L12, which are identical except that L7 is acetylated at the N terminus. It is a component of the L7/L12 stalk, which is located at the surface of the ribosome. The stalk base consists of a portion of the 23S rRNA and ribosomal proteins L11 and L10. An extended C-terminal helix of L10 provides the binding site for L7/L12. L7/L12 consists of two domains joined by a flexible hinge, with the helical N-terminal domain (NTD) forming pairs of homodimers that bind to the extended helix of L10. It is the only multimeric ribosomal component, with either four or six copies per ribosome that occur as two or three dimers bound to the L10 helix. L7/L12 is the only ribosomal protein that does not interact directly with rRNA, but instead has indirect interactions through L10. The globular C-terminal domains of L7/L12 are highly mobile. They are exposed to the cytoplasm and contain binding sites for other molecules. Initiation factors, elongation factors, and release factors are known to interact with the L7/L12 stalk during their GTP-dependent cycles. The binding site for the factors EF-Tu and EF-G comprises L7/L12, L10, L11, the L11-binding region of 23S rRNA, and the sarcin-ricin loop of 23S rRNA. Removal of L7/L12 has minimal effect on factor binding and it has been proposed that L7/L12 induces the catalytically active conformation of EF-Tu and EF-G, thereby stimulating the GTPase activity of both factors. In eukaryotes, the proteins that perform the equivalent function to L7/L12 are called P1 and P2, which do not share sequence similarity with L7/L12. However, a bacterial L7/L12 homolog is found in some eukaryotes, in mitochondria and chloroplasts. In archaea, the protein equivalent to L7/L12 is called aL12 or L12p, but it is closer in sequence to P1 and P2 than to L7/L12.
Statistics
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PSSM-Id: 100102
View PSSM: cd00387
Aligned: 128 rows
Threshold Bit Score: 69.8769
Threshold Setting Gi: 88608716
Created: 6-Mar-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 26 residues -Click on image for an interactive view with Cn3D
Feature 1:core dimer interface [polypeptide binding site]
Evidence:
  • Structure:1YL3_J; Thermus thermophilus ribosomal protein L7/L12 forms a dimer with another L7/L12 monomer (1YL3_I), defined using 3.5 A contacts
    View structure with Cn3D
  • Structure:1DD3_A; Thermotoga maritima ribosomal protein L7/L12 forms a dimer with another L7/L12 monomer (1DD3_B), defined using 3.5 A contacts
    View structure with Cn3D
  • Comment:The core dimer interface is formed mainly by interactions in the helical N-terminal domain (NTD). The NTD is also essential in binding the L10 helix.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        #            ###  ##  #  #                                                      
1YL3_J         1 MTIDEIIEAIEKLTVSELAELVKKLEDKFGVta----------------------------------------------- 33
gi 156084081  57 DRVIRLVDEILNLTLIESADLCDLCQERLAGpnvnggfipgrspfph--------------------------------- 103
gi 71031400   65 LKVIKLVDEILNLTLIEVADLCNLCQDKLSTnnvnnrlpfph-------------------------------------- 106
gi 145521640 110 KEKEQIEAYMKELFGENVPDFKAIFGGVGSIgs----------------------------------------------- 142
gi 146184118  90 KRVEFIADAFIELNIMEIRYLNHTLKERLIKttglsplkvnidwpslkqlengswppanpnwflqqeaiaklwpagqagl 169
gi 159487146  54 PKVQKLAEEIMGLTVLECSMLSEILRKKLGVqqpafsmg----------------------------------------- 92
gi 88608716    4 VECSEIAQKILSLSLVEAAELAAELKKVLNLpdv---------------------------------------------- 37
gi 32477743   15 AEAKELGDKIANMTLKQAKELSDYLKDEHGIepaag-------------------------------------------- 50
gi 87306546   12 EELKTLGDKIATLTLKQAVELGDYLKDAHGIeaa---------------------------------------------- 45
gi 149177089  12 EETKELGDKIAGLTLLQAKDLAEYLDEVHGIkaa---------------------------------------------- 45
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                               #  ## ###                            ##                  
1YL3_J        34 ------------------aaPVAVAAAPVAGAaagaa-------------------qeekTEFDVVLKSFGq-NKIQVIK 75
gi 156084081 104 ----phtffsgvgmmperlsPMGVMPQPVAATvetaepppae--------tpqptkpaqkSTGTLTLVGFDatKKIALIK 171
gi 71031400  107 ----------pnsffqyngfAGNMNFTPPPTPtnttdstdstpkntnvstkeepvkekvpVKRTIKLVGFDkeKKIDVIK 176
gi 145521640 143 -------------------qAPSQSAASSEAPkqeqkkaea-----------apkveaekKNYDVELSAIDaaQKIKIIK 192
gi 146184118 170 sqlfggmmgggaaggsgapqAAAAAAAPAAEKpkeea-------------------pkekQNYDVELSAIDaaQKIKIIK 230
gi 159487146  93 -------------ampfaaaPAAPAAAPAAAAapekke-----------------ekkekTEFDVKLESFSaeGKIKVIK 142
gi 88608716   38 -----------------amaPAAVAAAPATSEsvek--------------------kgeaSKFTLILKSYGe-KKTNAIK 79
gi 32477743   51 ----------------ggavMMAGPAGDGGGAaev-----------------------eqTEFDVVLTGFGe-KKLNVVK 90
gi 87306546   46 ------------------agGGVMMAGPAAGPaeea---------------------avqTEFDVVMTSFGg-NKISVIK 85
gi 149177089  46 ------------------agGAVMMAGPAAEAgpaa---------------------eekTEFDVILTGFGd-NKIPVIK 85
                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*...
Feature 1           # #      # # ##                ## #  #                 
1YL3_J        76 VVREIT------GLGLKEAKDLVEKAgspdAVIKSGVSKEEAEEIKKKLEEAGAEVEL 127
gi 156084081 172 LVRTIT------GLGLRESKELVESAp---RQLKKDLPLEEVQKLAQDLEAAGATIKI 220
gi 71031400  177 TIRTIL------NISLRESKELIESYp---KVIKKNVSEEELEKLTKLIKDSGGHVEV 225
gi 145521640 193 EVRQLL------NLGLKEAKDLVEKLp---ANLGKQVPKEKANELKEKLTAAGCTINL 241
gi 146184118 231 EVRTIL------NLGLKEAKDMVEKTp---CILKSACKKAEAEELKEKLAAIGCTINL 279
gi 159487146 143 EIRALT------TLGLKEAKELVEKAp---VVIKVGVSKADAEAMKKQLETAGGKVSY 191
gi 88608716   80 ALKTIFkqelgqDMGLKELKDKVEAVp---VDVLSGLTKEKADAYAKILKEAECEPEL 134
gi 32477743   91 VVKNIT------GASLMDAKKMVEGCp---ATLKEAVSKEDAEKVKAEIEEAGGSVEL 139
gi 87306546   86 AIRSIT------GLGLKEAKELTEGVp---SKVKEGVSKEDAEKVKAELTDAGAEVEI 134
gi 149177089  86 IVRAAT------GLGLKEAKDLVEGAp---KPLKEGISKEDAEALVKEVKEAGGTAEV 134

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