1GA8,1LL2,1LL0


Conserved Protein Domain Family
Glyco_transf_8

?
cd00505: Glyco_transf_8 
Click on image for an interactive view with Cn3D
Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis.
Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
Statistics
?
PSSM-Id: 132996
View PSSM: cd00505
Aligned: 8 rows
Threshold Bit Score: 213.457
Threshold Setting Gi: 74603159
Created: 6-Mar-2002
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
 
ligand bindingoligomermetal binding
Conserved site includes 22 residues -Click on image for an interactive view with Cn3D
Feature 1:ligand binding site
Evidence:
  • Structure:1GA8_A: Neisseria Lgtc binds Mn and UDP-2-deoxy-2-fluorogalactose, a donor sugar analog
    View structure with Cn3D
  • Structure:1GA8_A: Neisseria Lgtc binds 4'-deoxylactose, a sugar acceptor analog
    View structure with Cn3D
  • Comment:Donor and acceptor binding seems to be cooperative; in some glycosyltransferases the nucleotide-sugar bind first, and in others, the acceptor

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              ###  ##                                                                    
1GA8_A          1 XDIVFAADd-NYAAYLCVAAKSVEAAHpdteIRFHVLDAg------iSEANRAAVAAnlrgg-ggniRFIDVNPEdfagf 72
gi 50754115    67 ISVVIAASd-ERLGGAIAAMNSIYRNTrs-nVVFYIVTLndtvdhlrLWLRNTALKNlry----hilNFDPRVLEgkvqv 140
gi 51872295   139 IHIAIVCAgyNASRDVVTLVKSVLFHRrn-pLHFHIITDs------iARKILADLFHtwmvp-avhvNFYDADELksevs 210
1LL2_A          4 QAFVTLTTndAYAKGALVLGSSLKQHRt--sRRLAVLTTpq-----vSDTMRKALEIvf-------dEVITVDILdsgds 69
gi 24659591    85 MHLAVVACg-ERLEETMTMLKSAIIFSik-pLQFHIFAEdq-----lHHSFKGRLDNwsflq-tfnyTLYPITFPsenaa 156
gi 118094837 1230 LNIFSVASghLYERFLRIMMLSVLKHTkt-pLKFWFLKNy------lSPTFKEFIPYmakky-nfqyELVQYKWPrwlhq 1301
gi 74603159    68 YAYVQYATdfDYLNLAIINFIVLRRSStk-iPNLVVLFNr------aLQEDKNRFDGlrdlslrysvTLKPIPIIenvna 140
1LL0_A         10 QAFVTLTTndAYAKGALVLGSSLKQHRt--sRRLAVLTTpq-----vSDTMRKALEIvf-------dEVITVDILdsgds 75
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                         #               # ###                                           
1GA8_A         73 pln---irhisittYARLKLGEYia-dcDKVLYLDIDVLVRD------------------SLTPLWdtdl---gdnWLGA 127
gi 50754115   141 dpqk--adsikpltFARFYLPNLvp-haEKAIYVDDDVIVQD------------------DILELYntpl---kpgHAAA 196
gi 51872295   211 wipn--khysgihgLMKLVLTKTlpsdlEKVIVLDTDITFAT------------------DIAELWvvfhkfkgqqVLGL 270
1LL2_A         70 ahltlmkrpelgvtLTKLHCWSLtq--ySKCVFMDADTLVLA------------------NIDDLFer-------eELSA 122
gi 24659591   157 ewk----klfkpcaSQRLFLPLIlk-evDSLLYVDTDILFLR------------------PVDDIWsllkkfnstqIAAM 213
gi 118094837 1302 qte----kqriiwgYKILFLDVLfplavDKILFVDADQIVRT------------------DLKELRdfnl---dgaPYGY 1356
gi 74603159   141 es------ptwsksFTKFHIFNEvk--yDRIVYFDADSMLLHtqwndnssivnnesnvpeNLDELFtip----eiiDIAL 208
1LL0_A         76 ahltlmkrpelgvtLTKLHCWSLtq--ySKCVFMDADTLVLA------------------NIDDLFer-------eELSA 128
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           #                                                              ###            
1GA8_A        128 SIDlfverqegyk----------------------------------------qkigxadgeyYFNAGVLLINLkkwr-- 165
gi 50754115   197 FSDdcdsttnkvavrgagnqynyi-------------------gfldykketirklamkantcSFNPGVFVANLtew--- 254
gi 51872295   271 VENqsdwylgnlwk---------------------------------------nhrpwpalgrGFNTGVILLLLdrlrk- 310
1LL2_A        123 APDpgw-------------------------------------------------------pdCFNSGVFVYQPsvet-- 145
gi 24659591   214 APEheeprigwynr---------------------------------------farhpyygktGVNSGVMLMNMtrmrrk 254
gi 118094837 1357 TPFcdsrremdgyrfw----------------------------------ksgywashlagrkYHISALYVVDLkkf--- 1399
gi 74603159   209 PQAywltkhtkasgkikapdgkgyqdeitalineisksvndslvfeklpsllvqsqklnhrnnFFATHVMVVKPsnei-- 286
1LL0_A        129 APDpgw-------------------------------------------------------pdCFNSGVFVYQPsvet-- 151
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                    ##                             ##    
1GA8_A        166 ------------rhdIFKXSSEWveqykd---------vxqyqDQDILNGLFKgg--------vCYANSRFNFXPTNyaf 216
gi 50754115   255 ------------klqNITKQLEKwmtlnvaeelysrtlagsitTPPLLIVFYKq---------hSSIDPLWNVRHLGsna 313
gi 51872295   311 ----------lkweqMWRLTAERelmsml---------stslaDQDIFNAVIKqnp-----flvHQLPCYWNVQLSDhtr 366
1LL2_A        146 ------------ynqLLHVASEQgsf--------------dggDQGLLNTFFNswat---tdirKHLPFIYNLSSISiys 196
gi 24659591   255 yfkndmttvrlqwgdILMPLLKKykln------------itwgDQDLLNIVFFhnp-----eslFVFPCQWNYRPDHciy 317
gi 118094837 1400 ------------rkiAAGDRLRGqyqglsqd-----pnslsnlDQDLPNNMIHqv-------piKSLPQEWLWCETWcdd 1455
gi 74603159   287 ------------fneLKKYVHNPwlwslhkrh---alrnksdyDMEVLNKFIDnvlqenenfkvGILPHKVYGVLTGefr 351
1LL0_A        152 ------------ynqLLHVASEQgsf--------------dggDQGLLNTFFNswat---tdirKHLPFIYNLSSISiys 202
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|
Feature 1                                              # ##    #    
1GA8_A        217 xanwfasrht----------dplyrdrtntvxpVAVSHYCGp--AKPWHR 254
gi 50754115   314 gkry----------------------spqfvkaAKLLHWNGh--FKPWGR 339
gi 51872295   367 sekc-----------------------ykdvsdLKVIHWNSp--KKLRVK 391
1LL2_A        197 ylpa----------------------fkafganAKVVHFLGq--TKPWNY 222
gi 24659591   318 gsnc----------------------qeaeeggIFILHGNRg--VYHDDK 343
gi 118094837 1456 s----------------------------skkrAKTIDLCNnpmTKEPKL 1477
gi 74603159   352 ellhesfvseaqflpfitgelneqwrpieiirnIKLIHFSDspiPKPWEG 401
1LL0_A        203 ylpa----------------------fkafganAKVVHFLGq--TKPWNY 228

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap