Conserved Protein Domain Family
MECDP_synthase

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cd00554: MECDP_synthase 
Click on image for an interactive view with Cn3D
MECDP_synthase (2-C-methyl-D-erythritol-2,4-cyclodiphosphate synthase), encoded by the ispF gene, catalyzes the formation of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MEC) in the non-mevalonate deoxyxylulose (DOXP) pathway for isoprenoid biosynthesis. This pathway is present in bacteria, plants and some protozoa but is distinct from that used by mammals and Archaea. MECDP_synthase forms a homotrimer, carrying three active sites, each of which is formed in a cleft between pairs of subunits.
Statistics
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PSSM-Id: 100025
View PSSM: cd00554
Aligned: 114 rows
Threshold Bit Score: 122.168
Threshold Setting Gi: 51701594
Created: 31-Jul-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:CDP-binding sites
Evidence:
  • Structure:1UZH; Mycobacterium 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase with bound CDP
    View structure with Cn3D
  • Comment:enzyme forms a homotrimer with three active sites, each formed in the cleft between pairs of subunits

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2UZH_A         7 RVGLGTDVHPIe-------------------------aGRPCRLLCLEFDDa-dGCAGHSDGDVAAHALCDALLSAAGLG 60
3B6N_A         6 RIGQGYDIHQIrvgppedivadttadtaantadpnkqsFKRLTIGGVPVETi--SVLSHSDGDVIFHALVDALLGGMSCS 83
gi 7531236    17 RTGIGQDSHRFlpe----------------------ssTKPCILGGIIFDHc-pGFQANSDGDIIFHAICNAISSVTNKI 73
gi 153869634   4 KTGLGQDSHRFdfd----------------------nqMKRLMLGGVIFDGe-sPLQGNSDADVVLHALCNAISGITGIN 60
gi 118602739   4 NTGLGQDSHAFcd------------------------tNKPLVLAGVVFDYp-kGLDANSDGDVIFHSITNAISSITGVN 58
gi 118726692  14 KVGIGQDSHRFdfq----------------------nnSKKFILGGVIFDDv-tPLDGNSDADVVLHALTNAISGVTCVN 70
gi 110004191   2 RVGNSYDLHNLk-------------------------mGTGFYLGGILIPCq-yQVEAVSDGDILLHTISEAIIGALGLG 55
gi 26554474   17 KIGNGIDIHKIkk------------------------eKCKQRLAGLDFELd-yKIIAHSDGDIILHSISSAILGALSLQ 71
gi 51701557    6 RIGQGFDSHKLkt-----------------------kkNSRVFLGGIPVRTd-qQLIANSDGDVVLHALSDAVLGCGSFG 61
gi 156083925  39 RIGLGYDIHRLvgt---------------------gkgGKPFKLGGVLVDDskiFVVGHSDGDTLLHALADALLGATGCG 97
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        # #                                         ##  ####                        ### 
2UZH_A        61 DLGTIFGTdrpqwrGASGADMIRHVRGLVENagfvIGNATVQVIGNr-PKVGPRREEAQQVLSELVga---pVSVSATTT 136
3B6N_A        84 DLGTLFPDgspkykNKNSLSFLRYARLLLYKrnyaIANVDIIVIAEv-PKISPIREEIVRNISSALgisesqVSLKGKTH 162
gi 7531236    74 ILGKVADEllqtrgITDSGIYLEEALKSLKPnq-kISHVAITIEGSr-PKFLCKLSALRQNIAQVMnltptdIGITATSG 151
gi 153869634  61 ILGAVSDAlclkhgITDSRFYVQEALNYLKDw--rICHVSFSIECKv-PKITPKIPEMKATISELLgletqdIGITATTG 137
gi 118602739  59 VLGAKADVlck-qgVTDSAEYLRLAFNDLNHc--kIQHIAISIECLy-PKISPMIEKMKFNISKLLnitledVGITATTG 134
gi 118726692  71 ILGKVSDEmclergITDSSEYLREALKYLVDv--kVVHVSISIECSy-PKISPKIAEMRKSISELLgipencVGITATTG 147
gi 110004191  56 DLGEWFDEtn---kKINSQLILKKALTLMAEqnyqISNIDTTIIIDe-PRLQKHKKAIKTNLQKLLqisaekINLKATTT 131
gi 26554474   72 DLGTYFSDtdsknkNLDSLEILSMCLNELKKqdysISNVDITIICEy-IIFKDIKDQIKSNLEKLLnn--teISLKATRY 148
gi 51701557   62 DIGMYFDEndlsnkGLDSKTILNYCLKLIKKlkleFVNIDLTIFAQd-IRIDPIRFEIKSSLMKLTgc--nsVNVKAKSY 138
gi 156083925  98 DIGDYFSEldsinkDMDSRIILESAIKEASLrgyrPTNIDINIILErvRLGRRLKDAIKKALYDMLgsd-vmINVKAKTN 176
                        170       180
                 ....*....|....*....|
Feature 1                            
2UZH_A       137 DGLgltgrgeGLAAIATALV 156
3B6N_A       163 EQLgpvgqkkAIECFANALL 182
gi 7531236   152 EGLsdfgcgdGVQCFCVLTV 171
gi 153869634 138 EGLtdfgrgeGIQVLCIVTA 157
gi 118602739 135 EGLtefgkgnGIQVFSIITV 154
gi 118726692 148 EGLtafgqgkGVQAFCIVTA 167
gi 110004191 132 EQNfp----nIIQSYTTLLL 147
gi 26554474  149 EEDk-----nMIQVNTVLLI 163
gi 51701557  139 EEPk-----nEIACSCVVLM 153
gi 156083925 177 EGLdaigrgeAVSCQVIAHL 196

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