Conserved Protein Domain Family
GH18_chitinase-like

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cd00598: GH18_chitinase-like 
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The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model.
Statistics
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PSSM-Id: 119349
View PSSM: cd00598
Aligned: 57 rows
Threshold Bit Score: 78.1884
Threshold Setting Gi: 9955018
Created: 13-Dec-2003
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1NH6_A; Serratia marcescens chitinase A binds hexasaccharide
    View structure with Cn3D
  • Structure:1E6Z_A; Serratia marcescens chitinase B binds allosamidin
    View structure with Cn3D
  • Structure:3B9A_A; Vibrio harveyi chitinase A binds hexasaccharide
    View structure with Cn3D
  • Structure:1LL4_D; Coccidioides immitis chitinase 1 binds Allosamidin
    View structure with Cn3D
  • Structure:2UY2_A; Saccharomyces cerevisiae CTS1 endochitinase binds 2 glycerol molecules
    View structure with Cn3D
  • Structure:1HKK_A; Human macrophage chitotriosidase binds allosamidin
    View structure with Cn3D
  • Comment:Allosamidin is thought to resemble the structure of a reaction intermediate that is unique among the glycoside hydrolases.
  • Structure:1WAW_A; Human macrophage chitotriosidase binds argadin, a chitinase inhibitor
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          #                                #                                            
1HKK_A         4 VCYFTNwaqyrqgear--flpkdldpslCTHLIYAFAGmtnhqlst---------------------------------- 47
1NH6_A       137 VGSYFVewgvygrnf----tvdkipaqnLTHLLYGFIPicggngindslkeiegsfqalqrscqgredfkvsihdpfaal 212
2DSK_A        12 FAPYIDmslsvhkpl-----veyakltgTKYFTLAFILyssvyngpaw-------------------------------- 54
1NAR_A         5 FREYIGvkpnsttlhdf-pteiintetlEFHYILGFAIesyyesgkgtgt------------------------------ 53
gi 29539602   18 VICYYQtyypnngtdyistlplltndcgVSHIILAAIHindepgnitlnd------------------------------ 67
gi 21908038  131 MMGGYLddrfiqgsv-----vqtpyafyHWQCIDVFVYfshht------------------------------------- 168
gi 118370480  12 SIAYINsvnkwwgadi--iydsfglnsnYDIIILSFWLsqqgafdavklyetiy------------------------qn 65
gi 118354355  54 KIAYINqlsewdgpki--ldamglnspcYNVFILTFWMpgwvsdalsvwenist-------------------------y 106
gi 118379333  85 KIAYVDtmkswwgpsi--itdsfglnscYDIIILSFWLtygtadaanlyeniyt-------------------------y 137
gi 50258140  312 YVIYADrwlsamp--------dvselssFNRFILAFWEsnggavddvq-------------------------------- 351
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1HKK_A        48 ------tewndetlYQEFNGLKkmn---pkLKTLLAIGGwnfgtqkf--tdmvatanNRQTFVNSAIRFlrk------ys 110
1NH6_A       213 qkaqkgvtawddpyKGNFGQLMalkqahpdLKILPSIGGwtlsdpf----ffmgdkvKRDRFVGSVKEFlqt-----wkf 283
2DSK_A        55 -----agsiplekfVDEVRELRei-----gGEVIIAFGGavgpyl-------cqqasTPEQLAEWYIKVidt------yn 111
1NAR_A        54 ---feeswdvelfgPEKVKNLKrrh---peVKVVISIGGrgvntpf----dpaeenvWVSNAKESLKLIiqkysddsgnl 123
gi 29539602   68 ---hspddpryvplWAEMRVMQas-----gTKVMGMVGGaakgsy-------qrldgSVADFEAFYCPLrdmi---rtrn 129
gi 21908038  169 ---------vtippVGWTNTAHrh-----gVCVLGTFITewneggrlceaflagderSYQAVADRLVQItqf------fr 228
gi 118370480  66 lgteqfgcsnsevqKNILDLFHqk-----qKKLFIAAFGgtdfp---------tsqnPLDVANKLSDWIksn------py 125
gi 118354355 107 isttkygktdaeiqKKILELFHaq-----gKQLFISAFGgtdnp---------tgsdPVQIASQLADFVnrn------py 166
gi 118379333 138 lgtdqygknntevqKNISDLFHsk-----gKQLFISAFGstnf----------ptsqDPIAVANKLADWvqk-----npy 197
gi 50258140  352 ----awsswdegyrRQIIEEYHda-----gIAIMIAAFGstdqp--------ttsgaDAKQVAQKLASFvme------yn 408
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            # # #                                                                       
1HKK_A       111 FDGLDLDWEYPgsqgsp-----avdkerFTTLVQDLANAFqqeaqtsgkerlLLSAAVpagqtyvda--------gyevd 177
1NH6_A       284 FDGVDIDWLFPggkganpnlgspqdgetYVLLMKELRAMLdqlsve-tgrkyELTSAIsagkdkidk---------vayn 353
2DSK_A       112 ATYLDFDIEAGida------------dkLADALLIVQRERpw---------vKFSFTLpsdpgiglaggy---giietma 167
1NAR_A       124 IDGIDIHYEHIrsd------------epFATLMGQLITELkkddd---lninVVSIAPsennsshyq---------klyn 179
gi 29539602  130 LNGLDLDVEEDmsl------------dgIIRLIDRLKSDFgd--------efIITLAPvatamvrglrhlsgfdyralet 189
gi 21908038  229 FDGWLINIENSlsla---------avgnMPPFLRYLTTQLhrqvp---gglvLWYDSVvqsgqlkwqd------elnqhn 290
gi 118370480 126 IDGVDIDYEDNeailng------tgvqwLIAFQQQIRFRLgn--------kyIITHAPqapyfskqyyqg--ggyynlhk 189
gi 118354355 167 IDGVDIDYEDNdaivng------tgvtwLITFQKALAEKInnk-------niIFTHAPqapyfsrayydn--gayytieq 231
gi 118379333 198 IDGIDIDYEDNdavvng------sgiswLITFQKQLRARIgn--------nyLITHAPqapyfsrayynd--ggyykldq 261
gi 50258140  409 LDGVDIDYEDMsamnsa------qavswIVELQMELRNLLps--------pyIISHAPvapwftsandysd-gsyvsihq 473
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                     ##                                                       #         
1HKK_A       178 kiaqNLDFVNLMAYDfhgswekvtghnsplykrqeqsg--aaaslnvdaavqqwlqkgtpaskLILGMPTYgrsftlass 255
1NH6_A       354 vaqnSMDHIFLMSYDfygafdlknlghqtalnapawkp---dtayttvngvnallaqgvkpgkIVVGTAMYgrgwtgvng 430
2DSK_A       168 kkgvRVDRVNPMTMDyywtpsnaenaikvaenvf-----------rqlkqiypeksdeeiwkmIGLTPMIGvnddks--- 233
1NAR_A       180 akkdYINWVDYQFSNqqkpvstddafve----------------------ifkslekdyhphkVLPGFSTDpldtkhn-- 235
gi 29539602  190 argsKISWYNVQFYNgrghmlhpsvyd-------------------------tiihqgweaerIVIGLLTNpangsq--- 241
gi 21908038  291 rvffDSCDGFFTNYNwreehlermlgqagerradvyvgvdvfargnvvggrfdtdkslelirkHGFSVALFapgwvyecl 370
gi 118370480 190 qvgnSIDWYNIQFYNqgdsntydsfeklfvcsgpp---------fletsyreiiqinqipiekLVLGKPATkqdvynt-- 258
gi 118354355 232 evghLISWYNVQFYNqesdsydsyeklflnsgap----------fvntsyqeiiekdkipiskLVLGKPATpkdadnt-- 299
gi 118379333 262 qvgsLIDWYNVQFYNqgddsydsltklfvqsgsp----------fvgtsyseiiqndgipiskIYLGKPATtadlsnt-- 329
gi 50258140  474 qvgdSIDFYSVQFYNqgpdqyvscetlitdsgse----------wpstsvfeinshagvpldkIVIGKPLEpgsasn--- 540
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1HKK_A       256 sdtrvgapatgsgtpgpftkeggmlay----yevcswkgatkqriqdqkvpyifrdnqwvgfddveSFKTKVSYLKQk-- 329
1NH6_A       431 yqnnipftgtatgpvkgtwengivdyrqiasqfmsgewqytydataeapyvfkpstgdlitfddarSVQAKGKYVLDk-- 508
2DSK_A       234 -------------------------------------------------------------vftleDAQQLVDWAIQh-- 250
1NAR_A       236 ------------------------------------------------------------kitrdiFIGGCTRLVQTf-- 253
gi 29539602  242 ------------------------------------------------------------gyvpmeTISSVLANVLTky- 260
gi 21908038  371 ekkdffqnqdkfwgrleryl-----------------pthsicslpfvtsfclgmgarrvcygqeeAVGPWYHLSAQe-- 431
gi 118370480 259 ------------------------------------------------------------gyvnpyDLGKIIRYAEInlq 278
gi 118354355 300 ------------------------------------------------------------gyvepaELGKICTEAQQklg 319
gi 118379333 330 ------------------------------------------------------------gyvdpaTLGSLVYQAQTtlg 349
gi 50258140  541 ------------------------------------------------------------gymsasDLHQCVSEAKErg- 559
                        410
                 ....*....|....
Feature 1                #     
1HKK_A       330 -gLGGAMVWALDLD 342
1NH6_A       509 -qLGGLFSWEIDAD 521
2DSK_A       251 -kIGSLAFWSVDRD 263
1NAR_A       254 -sLPGVFFWNANDS 266
gi 29539602  261 psFGGVSGWEYFNA 274
gi 21908038  432 -iQPLFGEHRLGGD 444
gi 118370480 279 fqVPAFFIWQLKSD 292
gi 118354355 320 ynIPGFFIWEFIND 333
gi 118379333 350 lkVPGFFIWQLKND 363
gi 50258140  560 -wNAGVMFWEWSEE 572

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