Conserved Protein Domain Family
GH25_muramidase

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cd00599: GH25_muramidase 
Click on image for an interactive view with Cn3D
Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.
Statistics
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PSSM-Id: 119373
View PSSM: cd00599
Aligned: 45 rows
Threshold Bit Score: 90.8708
Threshold Setting Gi: 46015792
Created: 7-Mar-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           #                          #                                                 
1JFX_A         6 QGIDVShwqgsin---wssvksaGMSFAYIKATEGtny-----------------------kdDRFSANYTNAynag--- 56
gi 45272226    3 KMVDVFsgsprs------fatlpETDITMVKATQGigy-----------------------vnPACNIDYANAkaag--- 50
gi 166157380 774 YFIDISshngiisvaefnslkqqGIQGVVVKLTEGtsy-----------------------inPYASSQIANAraag--- 827
gi 193598897 341 VVIDLShwnvnvd---fgmaksnGTVAVIHKATQGirn-----------------------idSCYRTRRIAAekeg--- 391
gi 13476193   24 LVVDAYeynsidw---gqlatdkRIAAFINKASDGmpppyfcfgsdndvklckalwkrhavtrELFQTRRVVAkalg--- 97
gi 169185001  10 KIIDVShhqglin---waavksdGVQGAFIKATEGrti-----------------------ldDKLEVNSKGAaeag--- 60
gi 18310296   10 FGPDLNefsrdvn----fltlakNSDFIYLRASGSgtg-----------------------klRIDNKFLEFAkecrrlg 62
gi 196229017  24 SILSLShydertsd--fsgmqrgGLEAIIHEATYPaye-----------------------ddAKYAYRQAEAmrag--- 75
gi 150378412 159 VVLDPYkgnsidw---nmvktdpRVAAVIHKASQGmt------------------------sdQKYAERKKVAtklg--- 208
gi 52079879    4 KGIDVShwqgnin---wkkvagdGIKFAFIKATEGttl-----------------------qdNKFVTNISGAnavg--- 54
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             # #                                         # #                            
1JFX_A        57 iirGAYHFarpn--assGTAQADYFASNgggwsr------dnrtlPGVLDIEhnpsgamcyglsttqmRTWINDFHARYK 128
gi 45272226   51 kllGLYHYcag----gnPIAEADYFINNikny---------vgeaVLAVDWEgyqns-------swgnYNYVRQFVNRVH 110
gi 166157380 828 ikvSAYHYahyt-saagAQEEARYFANAarsfg-------leastVMVNDMEessm--------vnniNNNVQAWQDEMR 891
gi 193598897 392 llwGAYHFgtn----tnGVIQANHFLNQvgnt----------satLLVLNVEpykn--------nimtQKQVEDFIKTVQ 449
gi 13476193   98 mkwGAYHLarp----gnPVEQANNFLDFadpa----------pddLMALDIEgidps-------qwmsLDDAEEFVRQVH 156
gi 169185001  61 laiGFYHYahpe--nndPLVEAAWFANAlkgf---------aaqfPHVLDVEgkastv-----ggeqlSKWCLAWLQEVE 124
gi 18310296   63 ipcGAYHFakpskdldsAVIQADQFIDVlqqgfgd----gdygdlFPVLDVEtptdks----ltttelVNWIDRFRDRFE 134
gi 196229017  76 llwGAYHFgng----tdGRKQADHFISFvgakwvtghsasqpsgvLLVLDAEqnthyp-----ggnmnVEQAVRFIERVH 146
gi 150378412 209 yawGSYHLltt----anVTQQIDHYLSIvgde----------akeSRAIDVEclggttscqsgsfkvsFASIEAALRRVK 274
gi 52079879   55 iktGAYHFarfg-skseALAEARFFLSVankv---------hltyPLVLDLEvnqrnv-----sksvlTDAAVAFLREVE 119
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                 #                            #                              #          
1JFX_A       129 ARttrDVVIYTTASWwntctgsw------ngmaakspFWVAHwgvsapt---------vpsgfptWTFWQYSAtgrvg-- 191
gi 45272226  111 ELtgvWCMVYVSQSEirqvan----------cindcpLWVAYykysqplnwdyqgagfniapwevFTIHQFTGs------ 174
gi 166157380 892 RQgysNLIHYTMASWldirggqvy-----tarfginnFWVAHyakgytymtqe--eakslnyyanAAAWQYTSvssk--- 961
gi 193598897 450 NItnnFVMIYASYNTlknystp---------fllktpLWIALyntqlkl----------ppgwdkWVLWQYTDgkkglwp 510
gi 13476193  157 RRvgrFPVLYTNGKTaqyiadnry----kyrllsrlpLWYARykpdidvh-------fpmgnwqgYALWQFSAqancgrf 225
gi 169185001 125 RLtghPTMIYSGASFaktylnk---------qvasfpLWIAHygvetpm---------pnstwdkWCVFQYTSsgnvk-- 184
gi 18310296  135 EKtrrRLMLYTGLFFiglyddfkvp--gkgyplsdmpLWIAMytripsnpri----ppnvggwkrWTMWQFTDegkld-- 206
gi 196229017 147 ERtgiYPGLYSNENWlkkvfgdpsissnsretlkkcwLWIANyhkqpas----------tavwdrWTLWQYTGdgvcglp 216
gi 150378412 275 EKtghFPLLYANHSVattlsarwk----dssefadvrLWYARfkknvtdf--------ptgpwksYTLWQFSSeinctps 342
gi 52079879  120 KAg-yFAMIYSGKSFlenclde--------sklkpfaLWVARynnt---------------lgrhADIWQYSDcgrva-- 173
                        250
                 ....*....|....*....
Feature 1                      #    
1JFX_A       192 --------gvsgdVDRNKF 202
gi 45272226  175 ------------dMDRNMV 181
gi 166157380 962 ---------lshaLDENID 971
gi 193598897 511 h-----gvngvglCDRDKF 524
gi 13476193  226 rcp-yrvagtpnnIDVNVV 243
gi 169185001 185 --------giagkVDINAM 195
gi 18310296  207 --------gvgspVDLNWG 217
gi 196229017 217 rrsyptsflsmrkVERTIF 235
gi 150378412 343 scp-yrvpgtqrdMDLNIY 360
gi 52079879  174 --------gisgnVDMNIC 184

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