Conserved Protein Domain Family
AAT_like

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cd00609: AAT_like 
Click on image for an interactive view with Cn3D
Aspartate aminotransferase family. This family belongs to pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). Pyridoxal phosphate combines with an alpha-amino acid to form a compound called a Schiff base or aldimine intermediate, which depending on the reaction, is the substrate in four kinds of reactions (1) transamination (movement of amino groups), (2) racemization (redistribution of enantiomers), (3) decarboxylation (removing COOH groups), and (4) various side-chain reactions depending on the enzyme involved. Pyridoxal phosphate (PLP) dependent enzymes were previously classified into alpha, beta and gamma classes, based on the chemical characteristics (carbon atom involved) of the reaction they catalyzed. The availability of several structures allowed a comprehensive analysis of the evolutionary classification of PLP dependent enzymes, and it was found that the functional classification did not always agree with the evolutionary history of these enzymes. The major groups in this CD corresponds to Aspartate aminotransferase a, b and c, Tyrosine, Alanine, Aromatic-amino-acid, Glutamine phenylpyruvate, 1-Aminocyclopropane-1-carboxylate synthase, Histidinol-phosphate, gene products of malY and cobC, Valine-pyruvate aminotransferase and Rhizopine catabolism regulatory protein.
Statistics
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PSSM-Id: 99734
View PSSM: cd00609
Aligned: 342 rows
Threshold Bit Score: 93.56
Threshold Setting Gi: 27573773
Created: 6-Mar-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:pyridoxal 5'-phosphate (PLP) forms an internal aldimine bond (Schiff base linkage) with the catalytic residue lysine
  • Structure:1IJI_A; L-Histidinol Phosphate Aminotransferase catalytic residue (lys) and cofactor (PLP)
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1AJS_A       33 VNLGVGAYrtddcqpwvlpvvrkve---qriannsslnheylpilGLAEFRTCASRLAlgddspa-lqekrvGGVQSLGG 108
gi 14915801  31 ADLVIGAYrdqnglpyplkvvrkae----rrivdmgldkeyppmtGLLNFVEEAVKLAygntv----pleriAASQGLSG 102
gi 14915805  31 VNLGVGAYrdesgkpwilpavkeaea--iissdlskynkeyppvaGFPLFLEAAQFLMfgkdska-aqegriASCQSLSG 107
3TAT_A       29 VNLSIGLYynedgiipqlqavaeae---arlnaqphgaslylpmeGLNCYRHAIAPLLfgadhpv-lkqqrvATIQTLGG 104
gi 15794820  29 VNLSIGIYfddegkmpvlesvsrae----taraaapapspylpmeGLDTYRSAVQHLLfgkdnpa-laqgriATVQTLGG 103
gi 15605368  31 INLLLGTYerekkryggfssvrkaq----svffddekdknylpikGSSTFLEEMAALCfgevd-----asrwVGVQAIGG 101
1AY4_A       29 IDLGVGVYkdatghtpimravhaae----qrmletettktyaglsGEPEFQKAMGELIlgdgl----ksettATLATVGG 100
gi 20810274  30 PNKIFLAYrvcmtneghpwvslvvqktrlqisqdpslnyeylptmGLKSFIQASLALLfgkhsqa-ivenrvGGVHTVGD 108
gi 19703677  35 INATIGSLynedeklavydvvesvy----rnlppedlyayatnviGEDDYLEEVIKAVffddykealkelhiASIATTGG 110
gi 15639215  33 SSSEIVSYaptagipalrerwarr-----lcardpvlcpdepdvaAMQSAAEQGEHSCaavf------pkmsLPIVLPGL 101
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1AJS_A      109 TGALRIGAEFLarwyngtnnkdtPVYVSSPTWENHNGVFTTAgfkDIRSYrywdtekrgldl-----qgflsdlenapeF 183
gi 14915801 103 TGSLSLGATLLrqvv----pedtPVYVSNPTWSNHVSIFGIVghkNIREYryyspstheldf-----vgliedlnvapqG 173
gi 14915805 108 TGSLHIGFEFLhlwm-----pkaEFYMPSTTWPNHYGIYDKVf-nKLKVPykeytylrkdgeleidfsntkkdiqsapeK 181
3TAT_A      105 SGALKVGADFLkryf-----pesGVWVSDPTWENHVAIFAGAg-fEVSTYpwydeatngvrf-----ndllatlktlpaR 173
gi 15794820 104 SGALKVGADFLhrwf-----peaRAYVSDPTWDNHCGIFEGAg-fEVGTYpyydpatvgvkf-----demtaffntlpeN 172
gi 15605368 102 TGALHLGASVYanas-----lagKVYIPSQTWGNHSRIFAHQg-lALEYYpyydqetkeldl-----qglkailrsapeT 170
1AY4_A      101 TGALRQALELArman-----pdlRVFVSDPTWPNHVSIMNFMg-lPVQTYryfdaetrgvdf-----egmkadlaaakkG 169
gi 20810274 109 SGAFQLGVQFLrawh----kdarIVYIISSQKELHGLVFQDMgftVYEYSvwdpkklc-------------mdpdillnV 171
gi 19703677 111 TGAISNTVKNYmdt-------gdKVLLPNWMWGTYKNIVIENg-gKIETYqlfnengdfnfed----fknkvlelakiqK 178
gi 15639215 102 TAALSCAMDLFvga-------gqTVLVPAPRWENYDLMLAVRcaaTPVPFslfragrfdlsa-----ckealdaqasdgV 169
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1AJS_A      184 SIFVLHa---cAHNPTGTDptpeqwkqi-----asvmkrrfLFPFFDSAYQgfasgnlekdaw--airyfvsegfeLFCA 253
gi 14915801 174 SIIVLHa---cAHNPTGVDpskdqwati-----advfverkLIPFFDSAYQgfasgsldeday--airhfakrgmeMLLA 243
gi 14915805 182 SIFLFHa---cAHNPSGIDfteaqwkel-----lpimkekkHIAFFDSAYQgfatgsfeadaf--avrmfvdagveVLVA 251
3TAT_A      174 SIVLLHp---cCHNPTGADltndqwdav-----ieilkareLIPFLDIAYQgfgagmeeday---airaiasaglpALVS 242
gi 15794820 173 SVLILHp---cCHNPTGVDmseqqwdev-----lhiiktrkLIPFMDIAYQgfggdldsday---avrkavemelpLFVS 241
gi 15605368 171 SLVLLHc---cCHNPTGKDiplsewpei-----itiikerdLIPFFDMAYLgfasgieedrr---pvrlcieagvtTFVA 239
1AY4_A      170 DMVLLHg---cCHNPTGANltldqwaei-----asilektgALPLIDLAYQgfgdgleedaa---gtrliasripeVLIA 238
gi 20810274 172 VEQIPHgcvlvMGNIIDCKltpsgwakl-----msmikskqIFPFFDIPCQglytsgleedtr--ilqyfvsqgfeFFCS 244
gi 19703677 179 NVVLILne--pSHNPTGFRmtyeewvnll--dffksikdtnIIVIRDVAYFeyddrgeeetkklrklligmpknilFMYA 254
gi 15639215 170 IRLILNf----PHNPSGYTpteeeaqqlyevvyayadagcaVLVICDDAYSgfeyeaslmrgsffarfaqahknicALKI 245
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           #                                                                           
1AJS_A      254 QSFSKNfglyNERVGNLTVVakepd--------silrVLSQMQKIvrvtwsnpPAQGARIVARTLsdpelfh---ewtgn 322
gi 14915801 244 QSFSKNmglyAERVGVISAVvsdas--------rkeaVRSRLEVIarsyystpPVHGARIAHLVMsdkelra---eweqe 312
gi 14915805 252 QSFSKNfglyGERIGCLHVVhagvegsveknkalsaaMVSGMTLQirktwsmsAIHGAYIVQVIVhdkrllq---mfydn 328
3TAT_A      243 NSFSKIfslyGERVGGLSVMcedae--------aagrVLGQLKATvrrnysspPNFGAQVVAAVLndealka---swlae 311
gi 15794820 242 NSFSKNlslyGERVGGLSVVcpnke--------eadlVFGQLKFTvrriysspPAHGAYIAADVMnspelya---lwqne 310
gi 15605368 240 GGASKIfslyGSRVGFFGAIhqdkl--------dlnrILSFLEEQirgeysspAREGVAIVTSILsnpylrq---ewele 308
1AY4_A      239 ASCSKNfgiyRERTGCLLALcadaa--------trelAQGAMAFLnrqtysfpPFHGAKIVSTVLttpelra---dwmae 307
gi 20810274 245 QSLSKNfgiyDEGVGMLVVVavnnq--------qllcVLSQLEGLaqalwlnpPNTGARVITSILcnpallg---ewkqs 313
gi 19703677 255 FSLSKSlsiyGMRIGAQIAVssdee--------viqeFKDALPFScrttwsniPKGGMKLFATIMknpelk----anflk 322
gi 15639215 246 DGLTKEeyawGLRVGFLSCAglalgv------sqyqaFEKKVMGMiraslsctATLTQTLALRLLaeedepaglgsatar 319
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1AJS_A      323 vKTMADRILSMRSELRARLEalktpgtw----------nhitdqigMFSFTGln--------pkQVEYLINqkHIYLLPs 384
gi 14915801 313 lKEMVNRVRSMRQGVYEGLMklgtpgtw----------ehiinqkgMFSYMGls--------rpQCERLCEk-RVFVLPv 373
gi 14915805 329 vKEMSARIHRMRSLLHASLAkrktpgpgskg-----twdhiltaigMFTFTGlt--------peHVDYLKEkwSIYLVKa 395
3TAT_A      312 vEEMRTRILAMRQELVKVLStempernf----------dyllnqrgMFSYTGls--------aaQVDRLREefGVYLIAs 373
gi 15794820 311 vYMMRDRIRAMRQKLYDVLTaqipdrdf----------tyfikqrgMFGYTGls--------veQVRRLRDefAVYLLDs 372
gi 15605368 309 lNGIRQSLEEIRSSFVIAMRnvaghsfd-----------fiasqkgFFGYPGfs--------keQVLFLREelGIYTTAg 369
1AY4_A      308 lEAVRSGMLRLREQLAGELRdlsgsdrf----------gfvaehrgMFSRLGat--------peQVKRIKEefGIYMVGd 369
gi 20810274 314 lKEVVENIMLTKEKVKEKLQllgtpgsw----------ghiteqsgTHGYLGln--------sqQVEYLVRkkHIYIPKn 375
gi 19703677 323 eKQGYMNLLNERANIFLKEAkeenlkv-------------lpyksgFFVTVPvget-----vdkIIEDLESk-NIFVIKf 383
gi 15639215 320 eRHQFFQLMCARYREVQRVVrdltwsttecgaavplrveflpcnsgYFTCIRcigfsaetlrqyLLEQCGIg-TVAIDEq 398
                       410       420
                ....*....|....*....|....*
Feature 1                                
1AJS_A      385 ---GRINMCglttKNLDYVATSIHE 406
gi 14915801 374 ---GRANLAaltpSTMDFLVKSIDD 395
gi 14915805 396 --gGRMSMCglteSNCDYVAEAIHD 418
3TAT_A      374 ---GRMCVAglntANVQRVAKAFAA 395
gi 15794820 373 ---GRMCVAglntSNTDYVARAFAE 394
gi 15605368 370 ---GRFNLNgitdKNINRVTHGFAQ 391
1AY4_A      370 ---SRINIAglndNTIPILARAIIE 391
gi 20810274 376 ---GQINFScinaNNINYITEGINE 397
gi 19703677 384 dtgIRIGLCsvpkRKIKGLAKKIKD 408
gi 15639215 399 --hLRVAFSalelPAIERVLRAVVH 421

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