1N8P,1E5E,1CL2,1IBJ,1CS1,1I41,1IBJ,1Y4I,2CTZ,1PG8


Conserved Protein Domain Family
CGS_like

?
cd00614: CGS_like 
Click on image for an interactive view with Cn3D
CGS_like: Cystathionine gamma-synthase is a PLP dependent enzyme and catalyzes the committed step of methionine biosynthesis. This pathway is unique to microorganisms and plants, rendering the enzyme an attractive target for the development of antimicrobials and herbicides. This subgroup also includes cystathionine gamma-lyases (CGL), O-acetylhomoserine sulfhydrylases and O-acetylhomoserine thiol lyases. CGL's are very similar to CGS's. Members of this group are widely distributed among all three forms of life.
Statistics
?
PSSM-Id: 99738
View PSSM: cd00614
Aligned: 43 rows
Threshold Bit Score: 379.624
Threshold Setting Gi: 31077042
Created: 6-Mar-2002
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:pyridoxal 5'-phosphate (PLP) forms an internal aldimine bond (Schiff base linkage) with the catalytic residue lysine
  • Structure:1N8P; catalytic residue (lys) and cofactor (PLP)
    View structure with Cn3D

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1N8P_A       23 GSVIEPISLSTTFKQSspan----------pigtYEYSRSQNPNRENLERAVAALEn----AQYGLAFSSGSATTATILQ 88
1CL2_A       22 GAVNSVIQRASSLVFDsveakkha--trnrangeLFYGRRGTLTHFSLQQAMCELEg----GAGCVLFPCGAAAVANSIL 95
gi 12644453  30 AGLPEPVKHEPTLPGPvfaahfhl---pgdptgpYTYGRDENPTWTRLESAIGELEapgeaGVETLVFASGMAAISSVLF 106
gi 13471558  24 DPIPLPLTMASIFHTPgve------------tgvDQYGRYDNPTWRAVEHVLGHLE-----DAQCVSFPSGMGAISSVFF 86
gi 585479    28 APVNLPSVRASTVRFQslakledaqrrkaageraSTYGRMGMDTHAALEQVFAELEg----GTHCYLASSGLAGISMVFL 103
gi 14521111  28 KPLHDPLYITAVFKQVgdaypt-------drgteLKYSREENPTVRKLEDKLAELEg----SDNALAFNSGMSAISTLYF 96
gi 18313332  18 GALQPPIYLTALFRMEgealks-------drgfdLKYSREENPTLRPLEEVVANLEg----GVDSLAFNSGMAALSTVFF 86
gi 14601818  21 EPVVPPVNVSAIYVHPpgsfere-----yprvgdLKYGRENNPTVLGLEEALTSLEe----ARWTLAFNSGMAALSTLVL 91
gi 29377727  22 HPMVPELVQTSSFYFPtyeefmaa---sideknnYVYTRGTNPTTEILEKKIARLEr----GEKCKVFASGMGAISATLF 94
gi 15644026  18 KALSFPIFETTNFYFDsfdemska---lrngdyeFVYKRGSNPTTRLVEKKLAALEe----CEDARLVASGMSAISLSIL 90
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1N8P_A       89 SLp-qGSHAVSIg-DVYGGTHRYFTkvanahgVETSFTNdll-ndLPQLIke-nTKLVWIETPTNPTLKVTDIQKVADLI 164
1CL2_A       96 AFieqGDHVLMTn-TAYEPSQDFCSkilsklgVTTSWFDpligadIVKHLqp-nTKIVFLESPGSITMEVHDVPAIVAAV 173
gi 12644453 107 SQlraGDTAVLPd-DGYQALPLVRAqle-aygIEVRTAPtgr-daQLDVLd--gAKLLWIETPSNPGLDVCDVRRLVEAA 181
gi 13471558  87 ALlksGDRVLLPa-DGYHATRAMAErflkplgITCDTRPtp--tfLDGGFk--gYSLVFVETPSNPRLDICDISAIAKAV 161
gi 585479   104 SLlsaGEHALVAd-CAYGPVHELHEavlsrlgIDVTFFDak--adLASLVrp-tTRLIFAEAPGSLLFEMLDMPALARFA 179
gi 14521111  97 SLlegGDKVVLSm-EGYGTTIELAKllr-kfgVDVKLAYpsa-dsLIETIde-eTKLVLVETMTNPTLKVIDVPEVAKRC 172
gi 18313332  87 SLlsaDASVLTPm-EAYGATLRLLNale-kygVRMRKAYpdt-etYIEALkavkPNLAFFETITNPMLRVLDGPEIIKAA 163
gi 14601818  92 HEvsrGAKKVLLerLTYGSTRSLLEmlssitgIEVRLAGppw-edLLDLVc--wADLIIVESMANPTLRVPPLSGIYREA 168
gi 29377727  95 TLlqqGDHVLMVn-TIYGESVSFVQyle-kfgVSLTKVDvaeteeLFQFVqe-nTKVIYFESPSSQKFELLDLEKITALA 171
gi 15644026  91 HFlssGDHVVCVd-EAYSWAKKFFNylskkfdIEVSYVPpda-erIVEAItk-kTKLIYLESPTSMRMKVIDIRKVTEAA 167
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                             #                                         
1N8P_A      165 KkhaagqdVILVVDNTFLSPyisnPLNFGADIVVHSATKYINGHsDVVLGVLATNnkpLYERLQFLqnaigaIPSPFDAW 244
1CL2_A      174 Rsvv--pdAIIMIDNTWAAGvlfkALDFGIDVSIQAATKYLVGHsDAMIGTAVCNa-rCWEQLRENaylmgqMVDADTAY 250
gi 12644453 182 Hag----gALVAVDNTLATPlgqrPLELGADFSVASGTKQLTGHgDVLLGYVAGRdagAMAAVRRWrkivgaIPGPMEAW 257
gi 13471558 162 Heq----gGALVVDNTTMTPfgqrPLDFGADIVVSADTKAVNGHsDVLFGHVASRdadIISKVTNWramaggIPGPFEAW 237
gi 585479   180 Kqh----dLILATDNTWGSGyiyrPLTLGAQVSVIAGTKYVGGHsDLMLGAVVTNdeaIAKRLNRTqyalgySVSADDAW 255
gi 14521111 173 Rev----gAILVVDNTFVTPllykPLEDGASYVVHSLTKYIAGHnDVLGGAVLWNg-eFSRDLWEWrrrlgtIIQPFDAW 247
gi 18313332 164 Kdl----gAVVVVDNTFATPvlttPISYGADVVVHSATKYLAGHnDVVGGIAVVArgdLLEELWIWramlgsIMQPFEAF 239
gi 14601818 169 Gsc----gVRVVVDNTFATPiayrPLERGAHYSLESLTKYIAGHnDVVGGSLSGRveeDLEPLWNMrkilgtIMQPIDAY 244
gi 29377727 172 Qki----gAYTVIDNTWASPlfqhPLCHNVDLVIHSCSKYIGGHsDIVAGAVIGKaalVDQIFEHGhqalgaVNSPFNSW 247
gi 15644026 168 Gel----kIKTVIDNTWASPifqkPKLLGVDVVVHSATKYISGHgDVMAGVIAGDvedMKNIFVDEyknigpVLSPIEAW 243
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1N8P_A      245 LTHRGLKTlhLRVRQAALSANKIAEFLAAdkenvvAVNYpglkthpnYDVVLKqhrdalgGGMISFRikg--gaEAASKF 322
1CL2_A      251 ITSRGLRTlgVRLRQHHESSLKVAEWLAEhpq-vaRVNHpalpgskgHEFWKRdft--gsSGLFSFVlkkklnnEELANY 327
gi 12644453 258 LAHRSIATlqLRVDRQDSTALKVAEALRTrpe-itGLRYpglpddpsHKVASQqml--ryGCVVSFTlp---srARADRF 331
gi 13471558 238 LVHRGLETleVRFDRMCSSAETIAQRLKEhra-vrGLRFpglngdpsHNLSRAqme--rfGFLISFEla---seDKAEDF 311
gi 585479   256 LALRGVRTmpVRMAQHARHALEVCEFLQNrpe-vvRLYHpawpadpgHALWQRdcs--gsNGMLAVQlgl--spQAARDF 330
gi 14521111 248 MIERGMRTleVRFERQSENAMAIAEFLKDhpk-vrEVHYpglkddpyYKVARRlfkkdlyGGVVAFDlgs--gdNALAFL 324
gi 18313332 240 LTLRGLKTlfVRFEKQCKTAMAVAQFLEGhgk-vaEVIYpglsshphHATAKRlfg-dkfGAVVSFRikg--grDAVVRF 315
gi 14601818 245 LAWRGMKTlkARFEAQSRAAVEVAEWLEShrk-vkKVYYpglsshpdHELARReln-glyGAVVSFEieg--grEEVFKL 320
gi 29377727 248 LALRGIRTmpVRLAHQSQAIQTVLLALQKdpr-isRLYHpfvgnqqqQALAEKylt--gyGSLFAIDlkd-tdfERLKTF 323
gi 15644026 244 LILRGLRTleLRMKKHYENALVVSDFLMDhpk-vlEVNYpmnprspqYELASSqms--ggSGLMSFRlkt-dsaEKVKEF 319
                       330       340       350       360       370       380
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
Feature 1                                                                          
1N8P_A      323 ASSTRLFTLAESLGgiESLLEVPavmthggipkeareasgvfddLVRISVGIEDTDDLLEDIKQALK 389
1CL2_A      328 LDNFSLFSMAYSWGgyESLILANqpehiaairp--qgeidfsgtLIRLHIGLEDVDDLIADLDAGFA 392
gi 12644453 332 LDALRLVEGATSFGgvRSTAERRgrwggd----------avpegFIRLSVGAEDPDDLVADLLRALD 388
gi 13471558 312 INNCPLIEAATSFGgvHTSAERRskrgd-----------avppgFVRLSVGCEPVEELWKAIETSLN 367
gi 585479   331 VNALTLFGIGFSWGgfESLVQLVtpgelarhq----ywqggsdaLVRLHIGLESPADLIADLAQALD 393
gi 14521111 325 RGLKRIFPSPSLGGv-ESIATYPvksaaktldeeireilgitdgLIRLSVGLEPLDELIEDIDNALG 390
gi 18313332 316 FKSLRLITPGPSLGgvESNATYPvasaaspipederkilgitedLVRLSIGLEEAEDLIEDLDRALN 382
gi 14601818 321 LSRLKLVTPSPSFGgvETIISYPaisshsnldplerselgvtdgLLRLSVGLEDVSDIIDDLSQALS 387
gi 29377727 324 VNALTLVTIGVSWGgfESLALPVfkgnnlaav----qkrglspaHIRMYVGLEEPTSIIEDIQQALD 386
gi 15644026 320 VESLRVFRMAVSWGshENLVVPRvaygdc---------pkkdvnLIRIHVGLGDPEKLVEDLDQALK 377

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap